Camphene isolated from essential oil of Piper cernuum (Piperaceae) induces intrinsic apoptosis in melanoma cells and displays antitumor activity in vivo

被引：84

作者：

Girola, Natalia ^{[1
]}

Figueiredo, Carlos R. ^{[1
]}

Farias, Camyla F. ^{[1
]}

Azevedo, Ricardo A. ^{[2
]}

Ferreira, Adilson K. ^{[2
]}

Teixeira, Sarah F. ^{[2
]}

Capello, Tabata M. ^{[3
]}

Martins, Euder G. A. ^{[4
]}

Matsuo, Alisson L. ^{[5
]}

Travassos, Luiz R. ^{[1
]}

Lago, Joao H. G. ^{[3
]}

机构：

[1] Univ Fed Sao Paulo, Expt Oncol Unit UNONEX, BR-04023062 Sao Paulo, SP, Brazil

[2] Univ Fed Sao Paulo, Lab Tumor Immunol, Inst Biomed Sci, BR-04023062 Sao Paulo, SP, Brazil

[3] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, BR-04023062 Sao Paulo, SP, Brazil

[4] Univ Sao Paulo, Inst Biosci, BR-05508 Sao Paulo, SP, Brazil

[5] Univ Fed Sao Paulo, Interdept Grp Hlth Econ Grides, BR-04023062 Sao Paulo, SP, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 467卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

Camphene; Melanoma; Apoptosis; Antitumor; ENDOPLASMIC-RETICULUM STRESS; ANTICANCER; EXPRESSION; LIMONENE;

D O I：

10.1016/j.bbrc.2015.10.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural monoterpenes were isolated from the essential oil of Piper cernuum yell. (Piperaceae) leaves. The crude oil and the individual monoterpenes were tested for cytotoxicity in human tumor cell lineages and B16F10-Nex2 murine melanoma cells. In the present work we demonstrate the activity of camphene against different cancer cells, with its mechanism of action being investigated in vitro and in vivo in murine melanoma. Camphene induced apoptosis by the intrinsic pathway in melanoma cells mainly by causing endoplasmic reticulum (ER) stress, with release of Ca2+ together with HmgB1 and calreticulin, loss of mitochondrial membrane potential and up regulation of caspase-3 activity. Importantly, camphene exerted antitumor activity in vivo by inhibiting subcutaneous tumor growth of highly aggressive melanoma cells in a syngeneic model, suggesting a promising role of this compound in cancer therapy. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：928 / 934

页数：7

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