The CRISPR-Cas immune system: Biology, mechanisms and applications

被引：324

作者：

Rath, Devashish ^{[1
]}

Amlinger, Lina ^{[2
]}

Rath, Archana ^{[3
]}

Lundgren, Magnus ^{[2
]}

机构：

[1] Bhabha Atom Res Ctr, Div Mol Biol, Mumbai 400085, Maharashtra, India

[2] Uppsala Univ, Dept Cell & Mol Biol, SE-75124 Uppsala, Sweden

[3] Univ Mumbai, Dept Biotechnol, Mumbai 400098, Maharashtra, India

来源：

BIOCHIMIE | 2015年 / 117卷

基金：

瑞典研究理事会;

关键词：

CRISPR; Cascade; Cas9; Cas3; Cmr/Csm; SHORT PALINDROMIC REPEATS; GUIDED SURVEILLANCE COMPLEX; SEQUENCE-SPECIFIC ANTIMICROBIALS; ADAPTIVE BACTERIAL IMMUNITY; RNA-SILENCING COMPLEX; ESCHERICHIA-COLI; STREPTOCOCCUS-THERMOPHILUS; INTERFERENCE COMPLEX; CRYSTAL-STRUCTURE; MYCOBACTERIUM-TUBERCULOSIS;

D O I：

10.1016/j.biochi.2015.03.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Viruses are a common threat to cellular life, not the least to bacteria and archaea who constitute the majority of life on Earth. Consequently, a variety of mechanisms to resist virus infection has evolved. A recent discovery is the adaptive immune system in prokaryotes, a type of system previously thought to be present only in vertebrates. The system, called CRISPR-Cas, provide sequence-specific adaptive immunity and fundamentally affect our understanding of virus host interaction. CRISPR-based immunity acts by integrating short virus sequences in the cell's CRISPR locus, allowing the cell to remember, recognize and clear infections. There has been rapid advancement in our understanding of this immune system and its applications, but there are many aspects that await elucidation making the field an exciting area of research. This review provides an overview of the field and highlights unresolved issues. (C) 2015 The Authors. Published by Elsevier B.V.

引用

页码：119 / 128

页数：10

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