Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells

被引：23

作者：

Okamura, Daiji ^{[1
,2
]}

Maeda, Ikuma ^{[2
]}

Taniguchi, Hirofumi ^{[2
]}

Tokitake, Yuko ^{[2
]}

Ikeda, Makiko ^{[2
]}

Ozato, Keiko ^{[3
]}

Mise, Nathan ^{[4
]}

Abe, Kuniya ^{[4
]}

Noce, Toshiaki ^{[5
]}

Izpisua Belmonte, Juan Carlos ^{[1
,6
]}

Matsui, Yasuhisa ^{[2
]}

机构：

[1] Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA

[2] Tohoku Univ, Cell Resource Ctr Biomed Res, Inst Dev Aging & Canc, Sendai, Miyagi 9808575, Japan

[3] NICHHD, NIH, Lab Mol Growth Regulat, Bethesda, MD 20892 USA

[4] RIKEN, BioResource Ctr, Tsukuba, Ibaraki 3050074, Japan

[5] Keio Univ, Sch Med, Dept Physiol, Tokyo 1130021, Japan

[6] Ctr Regenerat Med Barcelona, Barcelona 08003, Spain

来源：

GENES & DEVELOPMENT | 2012年 / 26卷 / 22期

关键词：

primordial germ cell; CDK inhibitor; transcription; P-TEFb; 7SK snRNP; Larp7; BROMODOMAIN PROTEIN BRD4; P-TEFB; ELONGATION; EXPRESSION; REGULATORS; COMPONENT; CHROMATIN; COMPLEX; GROWTH; BINDS;

D O I：

10.1101/gad.202242.112

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15(INK4b), a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.

引用

页码：2477 / 2482

页数：6

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