Injectable hyperbranched poly(β-amino ester) hydrogels with on-demand degradation profiles to match wound healing processes

被引：134

作者：

Xu, Qian ^{[1
]}

Guo, Linru ^{[2
]}

Sigen, A. ^{[1
]}

Gao, Yongsheng ^{[1
]}

Zhou, Dezhong ^{[1
]}

Greiser, Udo ^{[1
]}

Creagh-Flynn, Jack ^{[1
]}

Zhang, Hong ^{[1
]}

Dong, Yixiao ^{[1
]}

Cutlar, Lara ^{[1
]}

Wang, Fagang ^{[3
]}

Liu, Wenguang ^{[2
]}

Wang, Wei ^{[1
,2
]}

Wang, Wenxin ^{[1
,2
]}

机构：

[1] Univ Coll Dublin, Sch Med, Charles Inst Dermatol, Dublin 4, Ireland

[2] Tianjin Univ, Tianjin Key Lab Composite & Funct Mat, Sch Mat Sci & Engn, Tianjin 300350, Peoples R China

[3] Shandong Univ, Shandong Prov Hosp, Dept Burn & Plast Surg, Jinan 250001, Shandong, Peoples R China

来源：

CHEMICAL SCIENCE | 2018年 / 9卷 / 08期

基金：

爱尔兰科学基金会; 中国国家自然科学基金; 国家杰出青年科学基金;

关键词：

GENE DELIVERY; POLYMER LIBRARY; TISSUE; NANOPARTICLES; SCAFFOLDS; THERAPY; GROWTH; DNA;

D O I：

10.1039/c7sc03913a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Adjusting biomaterial degradation profiles to match tissue regeneration is a challenging issue. Herein, biodegradable hyperbranched poly(beta-amino ester)s (HP-PBAEs) were designed and synthesized via "A2 + B4" Michael addition polymerization, and displayed fast gelation with thiolated hyaluronic acid (HA-SH) via a "click" thiol-ene reaction. HP-PBAE/HA-SH hydrogels showed tunable degradation profiles both in vitro and in vivo using diamines with different alkyl chain lengths and poly(ethylene glycol) diacrylates with varied PEG spacers. The hydrogels with optimized degradation profiles encapsulating ADSCs were used as injectable hydrogels to treat two different types of humanized excisional wounds - acute wounds with faster healing rates and diabetic wounds with slower healing and neo-tissue formation. The fast-degrading hydrogel showed accelerated wound closure in acute wounds, while the slow-degrading hydrogel showed better wound healing for diabetic wounds. The results demonstrate that the new HP-PBAE-based hydrogel in combination with ADSCs can be used as a well-controlled biodegradable skin substitute, which demonstrates a promising approach in the treatment of various types of skin wounds.

引用

页码：2179 / 2187

页数：9

共 30 条

[1] Parallel synthesis and biophysical characterization of a degradable polymer library for gene delivery [J].

Akinc, A ;

Lynn, DM ;

Anderson, DG ;

Langer, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (18) :5316-5323

[2] Late-Onset Inflammatory Adverse Reactions Related to Soft Tissue Filler Injections [J].

Alijotas-Reig, Jaume ;

Teresa Fernandez-Figueras, Maria ;

Puig, Lluis .

CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY, 2013, 45 (01) :97-108

[3]

Anderson D.G., 2003, ANGEW CHEMIE, V115, P3261, DOI DOI 10.1002/ANGE.200351244

[4] A combinatorial library of photocrosslinkable and degradable materials [J].

Anderson, Daniel G. ;

Tweedie, Catherine A. ;

Hossain, Naushad ;

Navarro, Sergio M. ;

Brey, Darren M. ;

Van Vliet, Krystyn J. ;

Langer, Robert ;

Burdick, Jason A. .

ADVANCED MATERIALS, 2006, 18 (19) :2614-+

[5] Structure/property studies of polymeric gene delivery using a library of poly(β-amino esters) [J].

Anderson, DG ;

Akinc, A ;

Hossain, N ;

Langer, R .

MOLECULAR THERAPY, 2005, 11 (03) :426-434

[6] Growth factors and cytokines in wound healing [J].

Barrientos, Stephan ;

Stojadinovic, Olivera ;

Golinko, Michael S. ;

Brem, Harold ;

Tomic-Canic, Marjana .

WOUND REPAIR AND REGENERATION, 2008, 16 (05) :585-601

[7] Inflammatory cytokine levels in chronic venous insufficiency ulcer tissue before and after compression therapy [J].

Beidler, Stephanie K. ;

Douillet, Christelle D. ;

Berndt, Daniel F. ;

Keagy, Blair A. ;

Rich, Prestort B. ;

Marston, William A. .

JOURNAL OF VASCULAR SURGERY, 2009, 49 (04) :1013-1020

[8] The global burden of diabetic foot disease [J].

Boulton, AJM ;

Vileikyte, L ;

Ragnarson-Tennvall, G ;

Apelqvist, J .

LANCET, 2005, 366 (9498) :1719-1724

[9] Influence of macromer molecular weight and chemistry on poly(β-amino ester) network properties and initial cell interactions [J].

Brey, Darren M. ;

Erickson, Isaac ;

Burdick, Jason A. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 85A (03) :731-741

[10]

Castleberry S. A., 2015, ADV MATER, P1809

← 1 2 3 →