Purification of α-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae

被引：35

作者：

Dhananjay, S. K. ^{[1
]}

Mulimani, V. H. ^{[1
]}

机构：

[1] Gulbarga Univ, Dept Biochem, Gulbarga, Karnataka, India

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 09期

关键词：

Aspergillus oryzae; tert-butanol; alpha-galactosidase; purification; three-phase partitioning;

D O I：

10.1007/s10529-008-9722-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

alpha-Galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and pH). alpha-Galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. The fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by SDS-PAGE. Both purified preparations showed electrophoretic homogeneity on SDS-PAGE.

引用

页码：1565 / 1569

页数：5

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