Minocycline markedly reduces acute visceral nociception via inhibiting neuronal ERK phosphorylation

被引:30
作者
Cho, Ik-Hyun [1 ]
Lee, Min Jung
Jang, Minhee
Gwak, Nam Gil
Lee, Ka Yeon
Jung, Hyuk-Sang
机构
[1] Kyung Hee Univ, Dept Anat, Coll Oriental Med, Seoul 130701, South Korea
关键词
Minocycline; Acute visceral pain; c-Fos; p-ERK; Writhes; ACTIVATED PROTEIN-KINASE; FOS-LIKE PROTEIN; SPINAL-CORD; MICROGLIAL ACTIVATION; ACETIC-ACID; C-FOS; INFLAMMATORY PAIN; MECHANICAL ALLODYNIA; GLIAL ACTIVATION; NEUROPATHIC PAIN;
D O I
10.1186/1744-8069-8-13
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Background: Minocycline prevents the development of neuropathic and inflammatory pain by inhibiting microglial activation and postsynaptic currents. But, how minocycline obviates acute visceral pain is unclear. The present study investigated whether minocycline had an any antinociceptive effect on acetic acid-induced acute abdominal pain after intraperitoneal (i.p.) administration of saline or minocycline 1 hour before acetic acid injection (1.0%, 250 mu l, i.p.). Results: Minocycline (4, 10, or 40 mg/kg) significantly decreased acetic acid-induced nociception (0-60 minutes post-injection) and the enhancement in the number of c-Fos positive cells in the T5-L2 spinal cord induced by acetic acid injection. Also, the expression of spinal phosphorylated extracellular signal-regulated kinase (p-ERK) induced by acetic acid was reduced by minocycline pre-administration. Interestingly, intrathecal introduction of PD98059, an ERK upstream kinase inhibitor, markedly blocked the acetic acid-stimulated pain responses. Conclusions: These results demonstrate that minocycline effectively inhibits acetic acid-induced acute abdominal nociception via the inhibition of neuronal p-ERK expression in the spinal cord, and that minocycline may have therapeutic potential in suppressing acute abdominal pain.
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页数:13
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