Simultaneous Detection of Haemophilus influenzae Type b Polysaccharide-Specific Antibodies and Neisseria meningitidis Serogroup A, C, Y, and W-135 Polysaccharide-Specific Antibodies in a Fluorescent-Bead-Based Multiplex Immunoassay

被引：46

作者：

de Voer, Richarda M. ^{[1
,2
]}

Schepp, Rutger M. ^{[1
]}

Versteegh, Florens G. A. ^{[3
]}

van der Klis, Fiona R. M. ^{[1
]}

Berbers, Guy A. M. ^{[1
]}

机构：

[1] Natl Inst Publ Hlth & Environm, Lab Infect Dis & Screening, NL-3720 BA Bilthoven, Netherlands

[2] Univ Med Ctr Utrecht, Dept Immunol, Utrecht, Netherlands

[3] Groene Hart Ziekenhuis, Dept Pediat, Gouda, Netherlands

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2009年 / 16卷 / 03期

关键词：

LINKED-IMMUNOSORBENT-ASSAY; TOXOID CONJUGATE VACCINE; FLOW-CYTOMETRIC ASSAY; BORDETELLA-PERTUSSIS; SERUM ANTIBODIES; TETANUS; DIPHTHERIA; QUANTITATION; VALIDATION; INFANTS;

D O I：

10.1128/CVI.00364-08

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R = 0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.

引用

页码：433 / 436

页数：4

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