Association Constants in the Bovine Serum Albumin/Human Serum Albumin-Tween 20 System in Aqueous Solutions

被引：5

作者：

Vlasova, I. M. ^{[1
,2
]}

Vlasov, A. A. ^{[1
]}

Grapendaal, G. R. ^{[1
]}

Saletskii, A. M. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Phys, Moscow 119991, Russia

[2] Russian Acad Sci, Ctr Theoret Problems Physicochem Pharmacol, Moscow 119991, Russia

来源：

RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A | 2018年 / 92卷 / 04期

关键词：

bovine serum albumin; human serum albumin; Tween; 20; fluorescence quenching; association constants; ROTATIONAL DIFFUSION; TRYPTOPHAN FLUORESCENCE; DENATURATION; BROMIDE;

D O I：

10.1134/S0036024418040325

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The association constants in the bovine serum albumin/human serum albumin-Tween 20 system are determined in aqueous solutions at pH 3.5-8.0 by means of nonpolarized fluorescence and protein fluorescence quenching. It is found that the most efficient association of serum albumin molecules with Tween 20 micelles occurs at pH 5.0 near the isoelectric points of proteins.

引用

页码：714 / 718

页数：5

共 14 条

[1] Characterization of a dimeric unfolding intermediate of bovine serum albumin under mildly acidic condition [J].

Brahma, A ;

Mandal, C ;

Bhattacharyya, D .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1751 (02) :159-169

[2]

Dawson R.M. C., 1991, DATA BIOCH RES

[3] Quenching of BSA intrinsic fluorescence by alkylpyridinium cations -: Its relationship to surfactant-protein association [J].

Díaz, X ;

Abuin, E ;

Lissi, E .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2003, 155 (1-3) :157-162

[4]

Gennis R., 1997, BIOMEMBRANES MOL STR

[5]

GRYZUNOV YA, 1994, BLOOD SERUM ALBUMIN

[6]

LEVSHIN LV, 1994, OPTICAL METHODS INVE, V1

[7]

Permyakov EA, 2003, METHOD INTRINSIC PRO

[8]

Schmidt W., 2005, Optical Spectroscopy in Chemistry and Life Sciences

[9]

Tinoko I., 2005, PHYS CHEM PRINCIPLES

[10] Complexation of serum albumins and triton X-100: Quenching of tryptophan fluorescence and analysis of the rotational diffusion of complexes [J].

Vlasova, I. M. ;

Vlasov, A. A. ;

Saletskii, A. M. .

RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A, 2016, 90 (07) :1479-1483

← 1 2 →