Cloning and nucleotide sequence of β-mannanase and cellulase genes from Bacillus sp 5H

The two genes for beta-mannanase and cellulase of Bacillus sp. 5H have been cloned in Escherichia coli JM 109 by a shotgun method, though the cellulase gene was not expressed in Bacillus sp. 5H. The nucleotide sequences of the beta-mannanase gene and the cellulase gene revealed open reading frames of 1,086 and 1,503 base pairs, respectively, coding for a proteins of M-r 40,803 Da (beta-mannanase) and 55,420 Da (cellulase). The deduced primary structure of beta-mannanase comprised 362 amino acids which had a mature protein of 336 amino acids and a signal peptide of 26 amino acids and that of cellulase comprised 501 amino acid residues.