VE1 immunohistochemical detection of the BRAF V600E mutation in thyroid carcinoma: a review of its usefulness and limitations

被引：17

作者：

Na, Jong-In ^{[1
]}

Kim, Jo-Heon ^{[1
]}

Kim, Hye-Jeong ^{[1
]}

Kim, Hee-Kyung ^{[2
]}

Moon, Kyung-Sub ^{[3
]}

Lee, Ji-Shin ^{[1
]}

Lee, Jae-Hyuk ^{[1
]}

Lee, Kyung-Hwa ^{[1
]}

Park, Jong-Tae ^{[4
]}

机构：

[1] Chonnam Natl Univ, Dept Pathol, Sch Med, Gwang Ju 501746, South Korea

[2] Chonnam Natl Univ, Dept Internal Med, Sch Med, Gwang Ju 501746, South Korea

[3] Chonnam Natl Univ, Dept Neurosurg, Sch Med, Gwang Ju 501746, South Korea

[4] Chonnam Natl Univ, Dept Forens Med, Sch Med, Gwang Ju 501746, South Korea

来源：

VIRCHOWS ARCHIV | 2015年 / 467卷 / 02期

关键词：

Human BRAF protein; Real-time polymerase chain reaction; Immunohistochemistry; Thyroid carcinoma; BRAF(V600E) MUTATION; PAPILLARY CARCINOMAS; ASPIRATION BIOPSIES; PATHOGENIC ROLE; CLONAL ORIGIN; MELANOMA; CANCERS; DNA; ASSOCIATION; PATHOLOGY;

D O I：

10.1007/s00428-015-1773-0

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The BRAF V600E mutation is a valuable prognostic factor in thyroid carcinoma despite lingering debate. Successful immunohistochemical (IHC) detection of the BRAF V600E mutation using a VE1 antibody was introduced recently. The objective of this study was to verify the usefulness of IHC detection of the BRAF V600E mutation in thyroid carcinoma using the VE1 antibody. IHC detection of BRAF V600E was performed on various thyroid carcinoma subtypes. IHC results were compared with those obtained from real-time polymerase chain reaction (PCR) detection. Discordant cases were re-examined using a direct sequencing method following nested PCR amplification. The BRAF V600E mutation was detected in 68 % (71/104) of papillary carcinoma cases and 78 % (7/9) of anaplastic carcinoma cases. The mutation was not detected in patients with follicular carcinoma (0/18) or in medullary carcinoma (0/21). The overall sensitivity and specificity of IHC using the VE1 antibody were 100 and 94 %, respectively, suggesting that molecular-based results were indeterminable in four VE1-positive cases. IHC using the VE1 antibody is a highly sensitive and specific method for BRAF V600E mutation detection and may represent a future replacement for DNA-based molecular tests.

引用

页码：155 / 168

页数：14

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