Facile synthesis of enzyme-inorganic hybrid nanoflowers and their application as an immobilized trypsin reactor for highly efficient protein digestion

被引：128

作者：

Lin, Zian ^{[1
]}

Xiao, Yun ^{[1
]}

Wang, Ling ^{[1
]}

Yin, Yuqing ^{[1
]}

Zheng, Jiangnan ^{[1
]}

Yang, Huanghao ^{[1
]}

Chen, Guonan ^{[1
]}

机构：

[1] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Deparment Chem, Minist Educ,Key Lab Anal & Detect Food Safety, Fuzhou 350116, Peoples R China

来源：

RSC ADVANCES | 2014年 / 4卷 / 27期

基金：

中国国家自然科学基金;

关键词：

PEPTIDE SEPARATION; MASS-SPECTROMETRY; GROWTH; DEVICE;

D O I：

10.1039/c4ra00268g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel kind of immobilized trypsin reactor based on enzyme-inorganic hybrid nanoflowers was first developed and can be applied to ultra fast, highly efficient proteome digestion.

引用

页码：13888 / 13891

页数：4

共 28 条

[1] Reproducibility of a solid-phase trypsin microreactor for peptide mapping by capillary electrophoresis [J].

Bonneil, E ;

Mercier, M ;

Waldron, KC .

ANALYTICA CHIMICA ACTA, 2000, 404 (01) :29-45

[2] Super-hydrophobic tin oxide nanoflowers [J].

Chen, AC ;

Peng, XS ;

Koczkur, K ;

Miller, B .

CHEMICAL COMMUNICATIONS, 2004, (17) :1964-1965

[3]

Chiu CY, 2011, NAT CHEM, V3, P393, DOI [10.1038/nchem.1025, 10.1038/NCHEM.1025]

[4] Membrane-based nanoscale proteolytic reactor enabling protein digestion, peptide separation, and protein identification using mass spectrometry [J].

Cooper, JW ;

Chen, JZ ;

Li, Y ;

Lee, CS .

ANALYTICAL CHEMISTRY, 2003, 75 (05) :1067-1074

[5] Enhanced proteolytic activity enzymes in photopolymerized of covalently bound sol gel [J].

Dulay, MT ;

Baca, QJ ;

Zare, RN .

ANALYTICAL CHEMISTRY, 2005, 77 (14) :4604-4610

[6] The proteomic reactor: A microfluidic device for processing minute amounts of protein prior to mass spectrometry analysis [J].

Ethier, Martin ;

Hou, Weimin ;

Duewel, Henry S. ;

Figeys, Daniel .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (10) :2754-2759

[7] Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification [J].

Gao, J ;

Xu, JD ;

Locascio, LE ;

Lee, CS .

ANALYTICAL CHEMISTRY, 2001, 73 (11) :2648-2655

[8]

Ge J, 2012, NAT NANOTECHNOL, V7, P428, DOI [10.1038/nnano.2012.80, 10.1038/NNANO.2012.80]

[9] Proteins in Mesoporous Silicates [J].

Hudson, Sarah ;

Cooney, Jakki ;

Magner, Edmond .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (45) :8582-8594

[10] Highly Efficient Enzyme Reactors Containing Trypsin and Endoproteinase LysC Immobilized on Porous Polymer Monolith Coupled to MS Suitable for Analysis of Antibodies [J].

Krenkova, Jana ;

Lacher, Nathan A. ;

Svec, Frantisek .

ANALYTICAL CHEMISTRY, 2009, 81 (05) :2004-2012

← 1 2 3 →