The rate of fluid shear stress is a potent regulator for the differentiation of mesenchymal stem cells

被引：20

作者：

Yue, Danyang ^{[1
]}

Zhang, Mengxue ^{[1
]}

Lu, Juan ^{[1
]}

Zhou, Jin ^{[1
]}

Bai, Yuying ^{[1
]}

Pan, Jun ^{[1
]}

机构：

[1] Chongqing Univ, Coll Bioengn, State & Local Joint Engn Lab Vasc Implants, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing, Peoples R China

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2019年 / 234卷 / 09期

关键词：

chondrogenic differentiation; growth factors; mechanical stimulation; osteogenic differentiation; stem cells; MARROW STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; OSTEOBLAST DIFFERENTIATION; SUBSTRATE; LINEAGE; PROLIFERATION; THERAPY;

D O I：

10.1002/jcp.28296

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have previously demonstrated that the rate of fluid shear stress (SS) can manipulate the fate of mesenchymal stem cells (MSCs) to osteogenic or chondrogenic cells. However, whether SS is comparable to other two means of induction medium and substrate stiffness that have been proven to be potent in differentiation control is unknown. In this study, we subjected MSCs to 1-7 days of osteogenic or chondrogenic chemical induction, or 1-4 days of 37 or 86kPa of substrate stiffness induction, followed by 20min of Fast SS (0-0) or Slow SS (0-2), which is a laminar FSS that linearly increased from 0 to 10dyn/cm (2) in 0 (Fast) or 2 min (Slow) and maintained at 10dyn/cm (2) for a total of 20min. We found that 20min of SS could compete with 5 days' chemical and 2 days' substrate stiffness inductions. Our study confirmed that SS is a powerful tool to control the differentiation of MSCs, which stressed the possible application in MSCs linage specification.

引用

页码：16312 / 16319

页数：8

共 37 条

[1] Marrow adipocytes inhibit the differentiation of mesenchymal stem cells into osteoblasts via suppressing BMP-signaling [J].

Abdallah, Basem M. .

JOURNAL OF BIOMEDICAL SCIENCE, 2017, 24 :1-10

[2] THE EFFECTS OF DEXAMETHASONE AND 1,25-DIHYDROXYVITAMIN D-3 ON OSTEOGENIC DIFFERENTIATION OF HUMAN MARROW STROMAL CELLS IN-VITRO [J].

BERESFORD, JN ;

JOYNER, CJ ;

DEVLIN, C ;

TRIFFITT, JT .

ARCHIVES OF ORAL BIOLOGY, 1994, 39 (11) :941-947

[3] High glucose prevents osteogenic differentiation of mesenchymal stem cells via lncRNA AK028326/CXCL13 pathway [J].

Cao, Boran ;

Liu, Ning ;

Wang, Wenbo .

BIOMEDICINE & PHARMACOTHERAPY, 2016, 84 :544-551

[4] Efficacy of Mesenchymal Stromal Cells for Fistula Treatment of Crohn's Disease: A Systematic Review and Meta-Analysis [J].

Cao, Yantian ;

Ding, Zhen ;

Han, Chaoqun ;

Shi, Huiying ;

Cui, Lianlian ;

Lin, Rong .

DIGESTIVE DISEASES AND SCIENCES, 2017, 62 (04) :851-860

[5] Differential growth factor control of bone formation through osteoprogenitor differentiation [J].

Chaudhary, LR ;

Hofmeister, AM ;

Hruska, KA .

BONE, 2004, 34 (03) :402-411

[6] THE EFFECTS OF MECHANICAL LOADING ON MESENCHYMAL STEM CELL DIFFERENTIATION AND MATRIX PRODUCTION [J].

Delaine-Smith, Robin M. ;

Reilly, Gwendolen C. .

VITAMINS AND HORMONES: STEM CELL REGULATORS, 2011, 87 :417-480

[7] Matrix elasticity directs stem cell lineage specification [J].

Engler, Adam J. ;

Sen, Shamik ;

Sweeney, H. Lee ;

Discher, Dennis E. .

CELL, 2006, 126 (04) :677-689

[8] The Immunoregulatory Activity of Mesenchymal Stem Cells: 'State of Art' and 'Future Avenues' [J].

Fierabracci, Alessandra ;

Del Fattore, Andrea ;

Muraca, Maurizio .

CURRENT MEDICINAL CHEMISTRY, 2016, 23 (27) :3014-3024

[9] Bio-adhesive surfaces to promote osteoblast differentiation and bone formation [J].

García, AJ ;

Reyes, CD .

JOURNAL OF DENTAL RESEARCH, 2005, 84 (05) :407-413

[10] Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells [J].

Hassan, Ghmkin ;

Bahjat, Mohammad ;

Kasem, Issam ;

Soukkarieh, Chadi ;

Aljamali, Majd .

CELLULAR & MOLECULAR BIOLOGY LETTERS, 2018, 23

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