Microarray gene profiling of laser-captured cells: A new tool to study atherosclerosis in mice

被引：10

作者：

Paul, Antoni ^{[1
]}

Yechoor, Vijay ^{[1
]}

Raja, Rajiv ^{[3
]}

Li, Lan ^{[2
]}

Chan, Lawrence ^{[1
,2
]}

机构：

[1] Baylor Coll Med, Dept Med, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA

[3] Mol Devices Corp, Sunnyvale, CA 94089 USA

来源：

ATHEROSCLEROSIS | 2008年 / 200卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Atherosclerosis; Gene expression; IVT; LCM; Microarrays;

D O I：

10.1016/j.atherosclerosis.2007.12.056

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Genetically modified mice susceptible to atherosclerosis are widely used in atherosclerosis research. Although the atherosclerotic lesions in these animals show similarities to those in humans, comprehensive expression profile analysis of these lesions is limited by their very small size. In this communication, we have developed an approach to analyze global gene expression in mouse lesions by a combination of (a) laser capture microdissection (LCM) to isolate RNA from specific lesions, and (b) an efficient RNA amplification method that reliably yields sufficient quantities of high quality cRNA for quantitative real-time PCR (qPCR), as well as for microarray analysis. The RNA passed multiple quality controls and the expression profile observed in lesional cells compared with the whole artery encompasses genes that are characteristic of a macrophage/foam cell population. We believe that this method represents a useful new tool for the unbiased analysis of global gene expression of specific sub-regions in atherosclerotic lesions in different rodent models. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：257 / 263

页数：7

共 30 条

[21] Mechanisms of disease - Atherosclerosis - An inflammatory disease [J].