Molecular Cloning, Characterization, and mRNA Expression of Two Cryptochrome Genes in Helicoverpa armigera (Lepidoptera: Noctuidae)

被引:26
作者
Yan, Shuo [1 ]
Ni, Hui [1 ]
Li, Huiting [1 ]
Zhang, Jing [1 ]
Liu, Xiaoxia [1 ]
Zhang, Qingwen [1 ]
机构
[1] China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China
关键词
Helicoverpa armigera; photoreceptor; circadian clock; clock genes; cryptochrome; CIRCADIAN CLOCK GENES; BLUE-LIGHT RECEPTOR; DROSOPHILA; CRY; EYE; RHYTHMS; PROTEIN; PLANTS;
D O I
10.1603/EC12290
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Light is a major environmental signal for insect circadian. In this study, we isolated two cryptochrome (cry) genes from Helicoverpa armigera (Hubner) by reverse transcription polymerase chain reaction and RACE-PCR strategies, designated as Ha-cry1 (GenBank accession GQ896502) and Ha-cry2 (GenBank accession GQ896503). Ha-CRY1 encoded a fly-like protein of 548 amino acids, while Ha-CRY2 encoded a mammal-like protein of 657 amino acids. Both of these proteins had two conserved domains: a DNA photolyase domain and a flavin adenine dinucleotide(FAD) binding seven domain, and alignment of the amino acid sequence indicated that there was a high degree of homology between the CRYs of H. armigera and other insects. Real-time polymerase chain reaction revealed that: 1) Ha-cry1 and Ha-cry2 mRNA expressions were neither organ-specific nor developmental-stage-specific. 2) Under the light D dark cycle (16: 8 L: D), Ha-cry1 abundance tended to increase during the day, then decrease in the night, whereas the expression pattern of Ha-cry2 was opposite. 3) The cyclings of Ha-cry1 and Ha-cry2 expression were disturbed by constant light and darkness. Our study has significant importance for the further study of the functions of the Ha-cry genes and potential control of the cotton bollworm.
引用
收藏
页码:450 / 462
页数:13
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