Nucleotide-Bearing Benzylidene-Tetrahydroxanthylium Near-IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions

被引：16

作者：

Kuba, Miroslav ^{[1
,2
]}

Kraus, Tomas ^{[1
]}

Pohl, Radek ^{[1
]}

Hocek, Michal ^{[1
,2
]}

机构：

[1] Czech Acad Sci, Inst Organ Chem & Biochem, Flemingovo Nam 2, Prague 16610 6, Czech Republic

[2] Charles Univ Prague, Fac Sci, Dept Organ Chem, Hlavova 8, Prague 12843 2, Czech Republic

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2020年 / 26卷 / 52期

关键词：

DNA; fluorescence; nucleotides; real-time PCR; REAL-TIME PCR; POLYMERASE SYNTHESIS; FLUORESCENT-PROBES; THIAZOLE ORANGE; MESSENGER-RNA; NUCLEOSIDE; GREEN; ASSAY; NUCLEOBASES; AZIDE;

D O I：

10.1002/chem.202003192

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thymidine triphosphate bearing benzylidene-tetrahydroxanthylium near-IR fluorophore linked to the 5-methyl group via triazole was synthesized through the CuAAC reaction and was used for polymerase synthesis of labelled DNA probes. The fluorophore lights up upon incorporation to DNA (up to 348-times) presumably due to interactions in major groove and the fluorescence further increases in the single-stranded oligonucleotide. The labelled dsDNA senses binding of small molecules and proteins by a strong decrease of fluorescence. The nucleotide was used as a light-up building block in real-time PCR for detection of SARS-CoV-2 virus.

引用

页码：11950 / 11954

页数：5

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