Transcriptome analysis provides insight into deltamethrin-induced fat accumulation in 3T3-L1 adipocytes

被引：7

作者：

Qi, Zexiu ^{[1
]}

Lin, Jie ^{[1
]}

Gao, Ruichang ^{[1
]}

Wu, Weize ^{[1
]}

Zhang, Yan ^{[1
]}

Wei, Xinyuan ^{[1
]}

Xiao, Xiang ^{[1
]}

Wang, Huili ^{[2
]}

Peng, Ye ^{[1
]}

Clark, John M. ^{[3
]}

Park, Yeonhwa ^{[4
]}

Sun, Quancai ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu Provinc, Peoples R China

[2] Hefei Univ Technol, Sch Food Sci & Engn, Hefei 230009, Anhui Province, Peoples R China

[3] Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA

[4] Univ Massachusetts, Dept Food Sci, Amherst, MA 01003 USA

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2022年 / 184卷

基金：

中国国家自然科学基金;

关键词：

Deltamethrin; Pyrethroid; Lipid metabolism; Adipogenesis; Transcriptome; DIET-INDUCED ADIPOSITY; INSULIN-RESISTANCE; GENE-EXPRESSION; AMPK-ALPHA; TISSUE; ADIPOGENESIS; EXPOSURE; MICE; INCREASES; CHANNELS;

D O I：

10.1016/j.pestbp.2022.105114

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, deltamethrin (a Type-II pyrethroid) has been reported to increase triglyceride (fat) accumulation in adipocytes, while its underlying molecular mechanism is not fully determined. The aim of this study was to further investigate the molecular mechanisms of deltamethrin induced fat accumulation in murine 3T3-L1 adi-pocytes. Consistent to previous reports, deltamethrin (10 mu M) significantly promoted adipogenesis in 3T3-L1 adipocytes. RNA sequencing (RNA-seq) results showed that 721 differentially expressed genes (DEGs) were identified after deltamethrin treatment, involving in 58 Functional groups of Gene Ontology (GO) and 255 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Several key functional groups regulating adipogenesis, such as fat cell differentiation (Igf1, Snai2, Fgf10, and Enpp1) and cytosolic calcium ion concentration (Nos1, Cxcl1, and Ngf) were significantly enriched. Collectively, these results suggest that the promotion of adipogenesis by deltamethrin was attributed to an obesogenic global transcriptomic response, which provides further un-derstanding of the underlying mechanisms of deltamethrin-induced fat accumulation.

引用

页数：7

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