Enzymatic Mineralization of Hydrogels for Bone Tissue Engineering by Incorporation of Alkaline Phosphatase

被引：75

作者：

Douglas, Timothy E. L. ^{[1
,2
]}

Messersmith, Philip B. ^{[3
,4
]}

Chasan, Safak ^{[5
]}

Mikos, Antonios G. ^{[6
]}

de Mulder, Eric L. W. ^{[7
]}

Dickson, Glenn ^{[8
]}

Schaubroeck, David ^{[9
]}

Balcaen, Lieve ^{[10
]}

Vanhaecke, Frank ^{[10
]}

Dubruel, Peter ^{[2
]}

Jansen, John A. ^{[1
]}

Leeuwenburgh, Sander C. G. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen Med Ctr, Dept Biomat, NL-6500 HB Nijmegen, Netherlands

[2] Univ Ghent, Dept Organ Chem, Polymer Chem & Biomat PBM Grp, B-9000 Ghent, Belgium

[3] Northwestern Univ, Dept Biomed Engn, Chem Life Proc Inst, Evanston, IL 60208 USA

[4] Northwestern Univ, Inst Bionanotechnol Med, Evanston, IL 60208 USA

[5] Middle E Tech Univ, Dept Mol Biol & Genet, TR-06531 Ankara, Turkey

[6] Rice Univ, Dept Bioengn, Houston, TX USA

[7] Radboud Univ Nijmegen Med Ctr, Dept Orthoped, NL-6500 HB Nijmegen, Netherlands

[8] Queens Univ Belfast, Ctr Canc Res & Cell Biol, Belfast BT9 7BL, Antrim, North Ireland

[9] IMEC, ELIS, Ctr Microsyst Technol CMST, B-9052 Ghent, Belgium

[10] Univ Ghent, Dept Analyt Chem, B-9000 Ghent, Belgium

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 08期

基金：

美国国家卫生研究院;

关键词：

biomaterials; biomineralization; composites; enzymes; hydrogels; GLYCOL) FUMARATE) HYDROGELS; COLLAGEN TYPE-I; CALCIUM-PHOSPHATE; SUBSTRATE STIFFNESS; HYDROXYAPATITE; DIFFERENTIATION; COMPOSITES; CELLS; RAT; METHACRYLATE;

D O I：

10.1002/mabi.201100501

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG?>?collagen?>?OPF. The mineral:polymer ratio decreases in the order collagen?>?cPEG?>?OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.

引用

页码：1077 / 1089

页数：13

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