Colorimetric detection of Listeria monocytogenes using one-pot biosynthesized flower-shaped gold nanoparticles

被引:22
|
作者
Du, Juan [1 ,2 ,3 ,4 ]
Singh, Hina [2 ]
Dong, Wei-jie [5 ]
Bai, Yan-hong [1 ,3 ,4 ]
Yi, Tae-Hoo [2 ]
机构
[1] Zhengzhou Univ Light Ind, Coll Food & Bioengn, Zhengzhou 450001, Henan, Peoples R China
[2] Kyung Hee Univ, Coll Life Sci, Dept Oriental Med Biotechnol, Global Campus,1732 Deokyoungdaero, Yongin 446701, Gyeonggi Do, South Korea
[3] Henan Key Lab Cold Chain Food Qual & Safety Contr, Zhengzhou, Henan, Peoples R China
[4] Henan Collaborat Innovat Ctr Food Prod & Safety, Zhengzhou, Henan, Peoples R China
[5] Isotope Res Inst Ltd, Henan Acad Sci, Zhengzhou 450001, Henan, Peoples R China
基金
国家重点研发计划;
关键词
Colorimetric assay; Flower-shaped gold nanoparticles; Listeria monocytogenes; Biosynthesis; One-pot; SURFACE-PLASMON RESONANCE; BISPHENOL-A; NANOSTARS; ADSORPTION; GROWTH; SIZE;
D O I
10.1016/j.snb.2018.03.067
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The present study described a novel fast, simple and green one-pot synthesis approach of flower-shaped gold nanoparticles (flower-shaped AuNPs) by using antioxidant plant extracts. The AuNPs synthesized by Alchemilla mollis extract showed maximum absorbance at 610 nm and were polydispersed flower shaped with average size (+/-SD) of 68.7 +/- 2.8 nm. We have explored a colorimetric sensing assay for rapid detecting Listeria monocytogenes by using single-stranded DNA (ssDNA) conjugated flower-shaped AuNPs. ssDNA were used as probe for DNA detection and the synthesized flower-shaped AuNPs were used as indicators. The limit of detection for hlyA gene and genomic DNA of Listeria monocytogenes were 48.4 ng and 100.4 ng, respectively. The proposed assay is fast and specific for Listeria monocytogenes detection, with the improved color change and stability. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:285 / 292
页数:8
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