Hyaluronic Acid/Chitosan Coacervate-Based Scaffolds

被引:45
作者
Acar, Ozge Karabiyik [1 ]
Kayitmazer, A. Basak [2 ]
Kose, Gamze Torun [1 ]
机构
[1] Yeditepe Univ, Dept Genet & Bioengn, TR-34755 Istanbul, Turkey
[2] Bogazici Univ, Dept Chem, TR-34342 Istanbul, Turkey
关键词
MESENCHYMAL STROMAL CELLS; COMPLEX COACERVATION; BONE-MARROW; BOVINE SERUM; CHONDROGENIC DIFFERENTIATION; POLYELECTROLYTE COMPLEXES; ARTICULAR-CARTILAGE; MOLECULAR-WEIGHT; GENE-EXPRESSION; IONIC-STRENGTH;
D O I
10.1021/acs.biomac.8b00047
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chitosan-chloride (CHI) and sodium hyaluronate (HA), two semiflexible biopolymers, self-assemble to form nonstoichiometric coacervates. The effect of counterions was briefly investigated by preparing HA/CHI coacervates in either CaCl2 or NaCl solutions to find only a small difference in their tendency to coacervate. Higher water content in coacervates within CaCl2 was attributed to the chaotropic nature of Ca2+ ions. This effect was also evidenced with smaller pore sizes for coacervates in NaCl. Besides, for coacervation of chitosan-glutamate (CHI-G) with HA, dynamic light scattering at different charge ratios indicated a wider coacervation region for the HA/CHI-G pair than the HA/CHI. This was attributed to the chaotropic and "soft" ion nature of glutamate compared to chloride as a counterion of chitosan. Positive zeta potential values for both coacervate suspensions were explained by the contribution of charge mismatch, chain semiflexibility, and intra- and intercomplex disproportionation. Finally, HA/CHI coacervates were used to encapsulate bone marrow stem cells. While cell viabilities in HA/CHI coacervates were remarkable up to 21 days, their well-spread morphology has proved that HA/CHI coacervates are promising scaffolds for cartilage tissue engineering.
引用
收藏
页码:1198 / 1211
页数:14
相关论文
共 108 条
[21]   Chitosan Scaffolds Containing Hyaluronic Acid for Cartilage Tissue Engineering [J].
Correia, Clara R. ;
Moreira-Teixeira, Liliana S. ;
Moroni, Lorenzo ;
Reis, Rui L. ;
van Blitterswijk, Clemens A. ;
Karperien, Marcel ;
Mano, Joao F. .
TISSUE ENGINEERING PART C-METHODS, 2011, 17 (07) :717-730
[22]   Cell interactions with three-dimensional matrices [J].
Cukierman, E ;
Pankov, R ;
Yamada, KM .
CURRENT OPINION IN CELL BIOLOGY, 2002, 14 (05) :633-639
[23]   Response of polyelectrolyte complexes to subsequent addition of salts with different cations [J].
Dautzenberg, H ;
Kriz, J .
LANGMUIR, 2003, 19 (13) :5204-5211
[24]   Hyaluronan: A simple polysaccharide with diverse biological functions [J].
Dicker, Kevin T. ;
Gurski, Lisa A. ;
Pradhan-Bhatt, Swati ;
Witt, Robert L. ;
Farach-Carson, Mary C. ;
Jia, Xinqiao .
ACTA BIOMATERIALIA, 2014, 10 (04) :1558-1570
[25]   Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].
Dominici, M. ;
Le Blanc, K. ;
Mueller, I. ;
Slaper-Cortenbach, I. ;
Marini, F. C. ;
Krause, D. S. ;
Deans, R. J. ;
Keating, A. ;
Prockop, D. J. ;
Horwitz, E. M. .
CYTOTHERAPY, 2006, 8 (04) :315-317
[26]  
Fan CJ, 2017, TISSUE ENG PART B-RE, V23, P451, DOI [10.1089/ten.teb.2016.0465, 10.1089/ten.TEB.2016.0465]
[27]   Cytocompatible in situ forming chitosan/hyaluronan hydrogels via a metal-free click chemistry for soft tissue engineering [J].
Fan, Ming ;
Ma, Ye ;
Mao, Jiahui ;
Zhang, Ziwei ;
Tan, Huaping .
ACTA BIOMATERIALIA, 2015, 20 :60-68
[28]   The Hydrogel Nature of Mammalian Cytoplasm Contributes to Osmosensing and Extracellular pH Sensing [J].
Fels, Johannes ;
Orlov, Sergei N. ;
Grygorczyk, Ryszard .
BIOPHYSICAL JOURNAL, 2009, 96 (10) :4276-4285
[29]   Immobilization of Coacervate Microcapsules in Multilayer Sodium Alginate Beads for Efficient Oral Anticancer Drug Delivery [J].
Feng, Chao ;
Song, Ruixi ;
Sun, Guohui ;
Kong, Ming ;
Bao, Zixian ;
Li, Yang ;
Cheng, Xiaojie ;
Cha, Dongsu ;
Park, Hyunjin ;
Chen, Xiguang .
BIOMACROMOLECULES, 2014, 15 (03) :985-996
[30]   SHEAR-RATE, CONCENTRATION, MOLECULAR-WEIGHT, AND TEMPERATURE VISCOSITY DEPENDENCES OF HYALURONATE, A WORMLIKE POLYELECTROLYTE [J].
FOUISSAC, E ;
MILAS, M ;
RINAUDO, M .
MACROMOLECULES, 1993, 26 (25) :6945-6951