N-linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells

被引：5

作者：

Fujiyama, Kazuhito ^{[1
]}

Tanaka, Ryoji ^{[1
]}

Terashima, Masaaki ^{[2
]}

Seki, Tatsuji ^{[1
]}

机构：

[1] Osaka Univ, Int Ctr Biotechnol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Kobe Coll, Sch Human Sci, Dept Biosphere Sci, Nishinomiya, Hyogo 6628505, Japan

来源：

PLANT BIOTECHNOLOGY | 2007年 / 24卷 / 02期

关键词：

Arabidopsis thaliana; glycoproteins; glycosylation; N-linked glycan structures; suspension-cultured cells;

D O I：

10.5511/plantbiotechnology.24.255

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The oligosaccharide structures of total glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells are determined. The N-linked sugar chains released by hydrazinolysis were labeled with 2-aminopyridine, and analyzed by a combination of HPLC, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and exoglucosidase digestion. The glycan structures determined were GlcNAcMan(3)FucXylGlcNAc(2) (8.5%), Man(3)FucXylGlcNAc(2) (70.0%), Man(3)XylGlcNAc(2) (13.7%), and GlcNAcMan(3)XylGlcNAc(2) (7.8%). All the glycan structures of glycoproteins from Arabidopsis MM2d cells used in this study contain typical plant bisecting either beta 1,2-xylose or alpha 1,3-fucose residues, showing that suspension-cultured MM2d cells have a glycosylation potential similar to that of tobacco BY2 suspension-cultured cells.

引用

页码：255 / 259

页数：5