N-linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells

被引：5
|
作者：
Fujiyama, Kazuhito [1 ]
Tanaka, Ryoji [1 ]
Terashima, Masaaki [2 ]
Seki, Tatsuji [1 ]
机构：
[1] Osaka Univ, Int Ctr Biotechnol, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
[2] Kobe Coll, Sch Human Sci, Dept Biosphere Sci, Nishinomiya, Hyogo 6628505, Japan
来源：
PLANT BIOTECHNOLOGY | 2007年 / 24卷 / 02期
关键词：
Arabidopsis thaliana; glycoproteins; glycosylation; N-linked glycan structures; suspension-cultured cells;
D O I：
10.5511/plantbiotechnology.24.255
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The oligosaccharide structures of total glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells are determined. The N-linked sugar chains released by hydrazinolysis were labeled with 2-aminopyridine, and analyzed by a combination of HPLC, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and exoglucosidase digestion. The glycan structures determined were GlcNAcMan(3)FucXylGlcNAc(2) (8.5%), Man(3)FucXylGlcNAc(2) (70.0%), Man(3)XylGlcNAc(2) (13.7%), and GlcNAcMan(3)XylGlcNAc(2) (7.8%). All the glycan structures of glycoproteins from Arabidopsis MM2d cells used in this study contain typical plant bisecting either beta 1,2-xylose or alpha 1,3-fucose residues, showing that suspension-cultured MM2d cells have a glycosylation potential similar to that of tobacco BY2 suspension-cultured cells.
引用
收藏
页码：255 / 259
页数：5
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