A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays

被引：28

作者：

Li, Shanshan ^{[1
]}

Zhou, Li ^{[2
]}

Yao, Yongpeng ^{[1
]}

Fan, Keqiang ^{[1
]}

Li, Zilong ^{[1
]}

Zhang, Lixin ^{[1
,2
]}

Wang, Weishan ^{[1
]}

Yang, Keqian ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China

[2] Anhui Univ, Inst Hlth Sci, Hefei 230601, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 01期

基金：

中国国家自然科学基金;

关键词：

URIC-ACID BIOSENSOR; MICROBIAL BIOSENSORS;

D O I：

10.1039/c6cc07244e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A wide range of chemicals can be sensed by allosteric transcription factors (aTFs) in bacteria. Herein, we report a biosensing platform by using isolated aTFs as recognition elements in vitro. Moreover, a general strategy to increase the sensitivity of the aTF-based biosensors is provided. As a proof-of-concept, we obtained by far the most sensitive uric acid and oxytetracycline biosensors by using aTF HucR and OtrR as recognition elements, respectively. As a large number of aTFs are present in bacteria, our work opens a novel route to develop sensitive aTF-based biosensors.

引用

页码：99 / 102

页数：4

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