Two Adjacent Nucleotide-Binding Site-Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance

被引:438
作者
Ashikawa, Ikuo [1 ]
Hayashi, Nagao [2 ]
Yamane, Hiroko [3 ]
Kanamori, Hiroyuki [3 ]
Wu, Jianzhong [4 ]
Matsumoto, Takashi [4 ]
Ono, Kazuko [5 ]
Yano, Masahiro [5 ]
机构
[1] Natl Inst Crop Sci, NARO, Tsukuba, Ibaraki 3058518, Japan
[2] Natl Inst Agrobiol Sci, Plant Dis Resistance Res Unit, Tsukuba, Ibaraki 3058602, Japan
[3] Inst Soc Technoinnovat Agr Forestry & Fisheries, Tsukuba, Ibaraki 3050854, Japan
[4] Natl Inst Agrobiol Sci, Plant Genome Res Unit, Tsukuba, Ibaraki 3058602, Japan
[5] Natl Inst Agrobiol Sci, QTL Genom Res Ctr, Tsukuba, Ibaraki 3058602, Japan
关键词
D O I
10.1534/genetics.108.095034
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The rice blast resistance gene Pikm was cloned by a map-based cloning strategy. High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval. Sequence analysis predicted two adjacently arranged resistance-like genes, Pikm1-TS and Pikm2-TS, within this candidate region. These genes encoded proteins with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) and were considered the most probable candidates for Pikm. However, genetic complementation analysis of transgenic lines individually carrying these two genes negated the possibility that either Pikm1-TS or Pikm2-TS alone was Pikm. Instead, it was revealed that transgenic lines carrying both of these genes expressed blast resistance. The results of the complementation analysis and an evaluation of the resistance specificity of the transgenic lines to blast isolates demonstrated that Pikm-specific resistance is conferred by cooperation of Pikm-TS and Pikm2-TS. Although these two genes are not homologous with each other, they both contain all the conserved motifs necessary for an NBS-LRR class gene to function independently as a resistance gene.
引用
收藏
页码:2267 / 2276
页数:10
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