Sequence determination of the Crimean-Congo hemorrhagic fever virus L segment

被引：84

作者：

Kinsella, E

Martin, SG

Grolla, A

Czub, M

Feldmann, H

Flick, R

机构：

[1] Canadian Sci Ctr Human & Anim Hlth, Natl Microbiol Lab, Special Pathogens Program, Winnipeg, MB R3E 3R2, Canada

[2] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB R3E 0W3, Canada

来源：

VIROLOGY | 2004年 / 321卷 / 01期

关键词：

Crimean-Congo hemorrhagic fever virus; Bunyaviridae; Dugbe;

D O I：

10.1016/j.virol.2003.09.046

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Crimean-Congo hemorrhagic fever (CCHF) virus is highly pathogenic for humans and remains the only Category A virus for which full sequence information is currently unavailable. In this study we completed CCHF genome characterization by determining the L segment sequence using Dugbe and CCHF virus-specific oligonucleotides. Sequence alignments revealed the presence of four previously described conserved regions in all Bunyaviridae polymerases. Interestingly, additional regions containing putative Ovarian Tumor (OTU)-like cysteine protease and helicase domains were identified in the L segments of CCHF and Dugbe viruses, suggesting an autoproteolytic cleavage process for nairovirus L proteins. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：23 / 28

页数：6

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