Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler

被引：30
作者：
Bier, FF
Scheller, FW
机构：
[1] Inst. Biochem. and Molec. Physiol., University of Potsdam, c/o Max-Delbrück-Centre, D-13125 Berlin
来源：
BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 6-7期
关键词：
DNA; oligonucleotides; hybridisation; gene probes; evanescent wave; grating coupler;
D O I：
10.1016/0956-5663(96)83300-8
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Hybridisation of nucleic acid oligomers to an immobilised target has been observed in real time using evanescent field technology. A biotinylated 24-mer with random sequence including the EcoRI recognition site was immobilised via streptavidin onto a grating coupler wave guide surface. Hybridisation of 22-mer, 15-mer and 8-mer was observed. Activity of restriction endonuclease EcoRI was visualised by measurement of the loss of bound DNA after incubation.
引用
收藏
页码：669 / 674
页数：6
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