Ultrastructural changes induced by acai (Euterpe oleracea Mart) in MCF-7 breast cancer cell line

被引：5

作者：

Custodio Neto da Silva, Marcos Antonio ^{[1
]}

Soares, Camila Simoes ^{[2
]}

Assuncao Borges, Katia Regina ^{[3
]}

Souza Wolff, Lais Araujo ^{[3
]}

Lacerda Barbosa, Maria Do Carmo ^{[3
]}

Brandao Nascimento, Maria Do Desterro Soares ^{[3
]}

de Carvalho, Joao Ernesto ^{[2
]}

机构：

[1] Univ Estadual Campinas, Postgrad Program Internal Med, Fac Med Sci, Rua Tessalia Vieira de Camargo, Campinas, Brazil

[2] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP, Brazil

[3] Fed Univ Maranhao UFMA, Dept Pathol, Nucleum Basic & Appl Immunol, Sao Luis, Maranhao, Brazil

来源：

ULTRASTRUCTURAL PATHOLOGY | 2022年 / 46卷 / 06期

关键词：

Ultrastructural changes; acai; MCF-7; autophagy; DUAL ROLE; AUTOPHAGY; EXTRACT; ROLES; ROS;

D O I：

10.1080/01913123.2022.2141404

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Acai (Euterpe oleracea Mart) is an Amazon plant with many biological properties. Previous report of this group evidenced autophagy induction after treatment with acai seed extract in MCF-7 breast cancer cell lines by acridine orange assay. The aim of this study was to evaluate the ultrastructural changes induced by acai seed extract in MCF-7 breast cancer cell lines. First, MCF- 7 breast cancer cell line viability was evaluated by MTT assay. Acridine orange assay showed increase in the acidic compartments, suggesting autophagolysosome formation. These cells were treated with 25 mu g/ml for 24 h and evaluated by transmission electron microscopy (MET). This analysis showed that acai seed extract induced autophagy, confirmed by autophagolysosome formation. Furthermore, acai seed extract increased the number of mitochondria, suggesting the enrollment of reactive oxygen species in autophagy.

引用

页码：511 / 518

页数：8

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