Fine mapping of the human endothelin-converting enzyme gene by fluorescent in situ hybridization and radiation hybrids

被引：9

作者：

Albertin, G

Rossi, GP

Majone, F

Tiso, N

Mattara, A

Danieli, GA

Pessina, AC

Palu, G

机构：

[1] UNIV PADUA HOSP,SCH MED,DEPT BIOL,I-35126 PADUA,ITALY

[2] UNIV PADUA HOSP,SCH MED,DEPT MICROBIOL,I-35126 PADUA,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 221卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0656

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the sequence of the human endothelin-1-converting enzyme (hECE-1) cDNA, we cloned a 1982 bp cDNA fragment and we amplified by PCR a 3' fragment located on the last exon (exon 19). Human metaphase chromosomes were studied by Fluorescent in Situ Hybridization (FISH) using the 1982 digoxigenated hECE-1 fragment as a probe and chromosome 1-specific probes. Twin spot signals on each of the two homologous chromosomes 1 were found by FISH on band p36. The results of monochromosomal hybrids confirmed that hECE-1 is on chromosome 1. Radiation hybrid mapping localized the hECE-1 gene 3.15 cR far from D1S2436 (WI-3177), at about 25 cM from the telomere of the short arm, possibly at the border between 1p36.3 and 1p36.2. (C) 1996 Academic Press, Inc.

引用

页码：682 / 687

页数：6

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