Structural and functional chromatin organization of the SUP35 gene in Saccharomyces cerevisiae yeast

被引：0

作者：

Ryabinkova, NA ^{[1
]}

Vodopyanova, LG ^{[1
]}

Samsonova, MG ^{[1
]}

Myasnikova, EM ^{[1
]}

Osipova, TN ^{[1
]}

机构：

[1] ST PETERSBURG STATE TECH UNIV,DEPT APPL MATH,ST PETERSBURG 199064,RUSSIA

来源：

GENETIKA | 1997年 / 33卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Structural and functional organization of the 5' region of the SUP35 gene was analyzed in Saccharomyces cerevisiae yeast. Indirect DNA end labeling allowed two nuclease-hypersensitive sites and a region involved in nucleosomes to be revealed. DNase I and micrococcal nuclease hypersensitive sites were localized to almost the same regions: -461 ... -372 bp and -271 ... -91 bp for DNase I and -461 ... -356 bp and -231 ... -79 for micrococcal nuclease. Nucleosomes were localized to a region +22 ... +339 bp. Both the location of DNase I and micrococcal nuclease hypersensitive sites within the promoter region and the location of nucleosomes within the coding region remain the same at different cell culture growth phases. However, positioning nucleosomes were revealed within the SUP35 coding region only at the late logarithmic phase; the radioautographic pattern of them does not depend on the extent of nuclease digestion.

引用

页码：451 / 457

页数：7

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