Maintenance of steroid 11-hydroxylation activity in immobilized Cunninghamella elegans protoplasts

被引：17

作者：

Dlugonski, J ^{[1
]}

Paraszkiewicz, K ^{[1
]}

Sedlaczek, L ^{[1
]}

机构：

[1] POLISH ACAD SCI,CTR MICROBIOL & VIROL,PL-93231 LODZ,POLAND

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1997年 / 13卷 / 04期

关键词：

2-deoxy-D-glucose; hydroxylation; immobilization; polyoxin; protoplasts; steroids;

D O I：

10.1023/A:1018540620234

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cunninghamella elegans protoplasts entrapped in calcium alginate at gel concentration of 6% w/v maintained cortexolone 11 alpha- and 11 beta-hydroxylation activity for 13 cycles, and a total time of 247 h, in the presence of organic osmotic stabilizers and cell wall regeneration inhibitors. Changes in stabilization medium composition showed that it is possible to stimulate separately epihydrocortisone and hydrocortisone production in immobilized C. elegans protoplasts.

引用

页码：469 / 473

页数：5

共 12 条

[1] DLUGONSKI J, 1988, ACTA MICROBIOL POL, V37, P53
[2] DLUGONSKI J, 1984, CAN J MICROBIOL, V30, P57
[3] DLUGONSKI J, 1992, WORLD J MICROB BIOT, V8, P500
[4] STABILIZATION OF MICROBIAL CYTOCHROME-P-450 ACTIVITY BY CREATION OF STATIONARY-PHASE CONDITIONS IN A CONTINUOUSLY OPERATED IMMOBILIZED-CELL REACTOR
DROR, Y
FREEMAN, A
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (03) : 855 - 859
[5] TRANSFORMATION OF STEROIDS BY FUNGAL SPORES .3. ACTIVITY OF THE 11-HYDROXYLASES AT VARIOUS TIMES DURING GERMINATION AND VEGETATIVE GROWTH OF CUNNINGHAMELLA-ELEGANS SPORANGIOSPORES
JAWORSKI, A
SEDLACZEK, L
DLUGONSKI, J
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1984, 20 (05) : 313 - 317
[6] KOMEL R, 1985, APPL MICROBIOL BIOT, V23, P106
[7] NECS, 1985, FUNGAL PROTOPLASTS A, P115
[8] PEBERDY JF, 1991, MORE GENE MANIPULATI, P307
[9] APPLICATION OF IMMOBILIZED CELLS FOR BIOTRANSFORMATIONS OF STEROIDS
SCHMAUDER, HP
SCHLOSSER, D
GUNTHER, T
HATTENBACH, A
SAUERSTEIN, J
JUNGNICKEL, F
AUGSTEN, H
[J]. JOURNAL OF BASIC MICROBIOLOGY, 1991, 31 (06) : 453 - 477
[10] TRANSFORMATION OF STEROIDS BY FUNGAL PROTOPLASTS
SEDLACZEK, L
DLUGONSKI, J
JAWORSKI, A
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1984, 20 (03) : 166 - 169

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