Maintenance of steroid 11-hydroxylation activity in immobilized Cunninghamella elegans protoplasts

被引：17

作者：

Dlugonski, J ^{[1
]}

Paraszkiewicz, K ^{[1
]}

Sedlaczek, L ^{[1
]}

机构：

[1] POLISH ACAD SCI,CTR MICROBIOL & VIROL,PL-93231 LODZ,POLAND

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1997年 / 13卷 / 04期

关键词：

2-deoxy-D-glucose; hydroxylation; immobilization; polyoxin; protoplasts; steroids;

D O I：

10.1023/A:1018540620234

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cunninghamella elegans protoplasts entrapped in calcium alginate at gel concentration of 6% w/v maintained cortexolone 11 alpha- and 11 beta-hydroxylation activity for 13 cycles, and a total time of 247 h, in the presence of organic osmotic stabilizers and cell wall regeneration inhibitors. Changes in stabilization medium composition showed that it is possible to stimulate separately epihydrocortisone and hydrocortisone production in immobilized C. elegans protoplasts.

引用

页码：469 / 473

页数：5

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