Characterization of the distribution of rotational torque on electrorotation chips with 3D electrodes

被引:8
作者
Bahrieh, Garsha [1 ,3 ]
Ozgur, Ebru [3 ]
Koyuncuoglu, Aziz [3 ]
Erdem, Murat [2 ]
Gunduz, Ufuk [2 ]
Kulah, Haluk [1 ,3 ]
机构
[1] METU, Dept Elect & Elect Engn, Ankara, Turkey
[2] METU, Dept Biol, Ankara, Turkey
[3] METU MEMS Res & Applicat Ctr, Ankara, Turkey
关键词
Cell dielectric characterization; Electrorotation; Rotational torque; Rotating electric field; 3D electrodes; IMPEDANCE SPECTROSCOPY; DIELECTRIC-PROPERTIES; CELLS; DIELECTROPHORESIS; SEPARATION;
D O I
10.1002/elps.201500061
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This is a study of in-plane and out-of-plane distribution of rotational torque (ROT-T) and effective electric field (EEF) on electrorotation (ER) devices with 3D electrodes using finite element modeling (FEM) and experimental method. The objective of this study is to investigate electrical characteristics of the ER devices with five different electrode geometries and obtain an optimum structure for ER experiments. Further, it provides a comparison between characteristics of the 3D electrodes and traditionally used 2D electrodes. 3D distributions of EEF were studied by the time-variant FEM. FEM results were verified experimentally by studying the rotation of biological cells. The results show that the variations of ROT-T and EEF over the measurement area of the devices are considerably large. This can potentially lead to misinterpretation of recorded data. Therefore, it is essential to specify the boundaries of the measurement area with minimum deviation from the central EEF. For this purpose, FE analyses were utilized to specify the optimal region. Thereby, with confining the measurements to these regions, the dependency of ROT-T on the spatial position of the particles can be eliminated. Comparisons have been made on the sustainability of the EEF and ROT-T distributions for each device, to find an optimum design. Analyses of the devices prove that utilization of the 3D electrodes eliminate irregularities of EEF and ROT-T along the z-axis. The Results show that triangular electrodes provide the highest sustainability for the in-plane ROT-T and EEF distribution, while the oblate elliptical and circular electrodes have the lowest variances along the z-axis.
引用
收藏
页码:1785 / 1794
页数:10
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