Overexpression, crystallization and preliminary X-ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis

被引：2

作者：

Zhang, Liang ^{[1
]}

Chen, Ruyi ^{[1
]}

Dong, Zhe ^{[1
]}

Li, Xin ^{[1
]}

机构：

[1] Nankai Univ, State Key Lab Med Chem Biol, Coll Life Sci, Tianjin 300071, Peoples R China

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2013年 / 69卷

基金：

中国国家自然科学基金;

关键词：

PROTEIN DATA-BANK; NERVE AGENTS; CRYSTALS; CLONING;

D O I：

10.1107/S1744309112049032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Organophosphates (OPs) are extremely toxic compounds that are used as insecticides or even as chemical warfare agents. Phosphotriesterases (PHPs) are responsible for the detoxification of OPs by catalysing their degradation. Almost 100 PHP structures have been solved to date, yet the crystal structure of the phosphotriesterase from Mycobacterium tuberculosis (mPHP) remains unavailable. This study reports the first crystallization of mPHP. The crystal belonged to space group C222(1), with unit-cell parameters a = 68.03, b = 149.60, c = 74.23 angstrom, alpha = beta = gamma = 90 degrees. An analytical ultracentrifugation experiment suggested that mPHP exists as a dimer in solution, even though one molecule is calculated to be present in the asymmetric unit according to the structural data.

引用

页码：57 / 60

页数：4

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