Epigenetic modifications and mRNA levels of the imprinted gene Grb10 in serially passaged fibroblast cells

被引：4
作者：
Yao, Jianfeng [1 ,3 ]
Huang, Yanfang [2 ]
Huang, Rongfu [4 ]
Shi, Runhai [3 ]
Chen, Peng [2 ]
Zhu, Baoqin [1 ,5 ]
Li, Ming [1 ,5 ]
Jiang, Xiaohong [1 ,5 ]
Zheng, Ming [5 ]
Jiang, Yiping [1 ,6 ]
Yang, Xiaoyu [1 ,5 ,6 ]
机构：
[1] Fujian Med Univ, Ctr Cell & Dev Biol, Fuzhou 350004, Peoples R China
[2] Fujian Med Univ, Affiliated Hosp 1, Fuzhou 350004, Peoples R China
[3] QuanZhou Womens & Childrens Hlth Hosp, Quanzhou, Peoples R China
[4] Fujian Med Univ, Affiliated Hosp 2, Quanzhou, Peoples R China
[5] Fujian Med Univ, Dept Anat Histol & Embryol, Coll Preclin Med, Fuzhou 350004, Peoples R China
[6] Fujian Med Univ, Key Lab Stem Cell & Regenerat Med, Fuzhou 350004, Peoples R China
来源：
BIOCHIMIE | 2012年 / 94卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Grb10; Epigenetic modification; Fibroblasts; MOUSE PROXIMAL CHROMOSOME-11; NUCLEAR TRANSFER; HISTONE ACETYLATION; ALLELIC EXPRESSION; METHYLATION; FETAL; CLONING; TRANSCRIPTION; OOCYTES; BINDING;
D O I：
10.1016/j.biochi.2012.08.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The imprinted gene Grb10 influences fetal growth, adult behavior and tumor formation. Investigating this gene often involves consecutive passaging of cells in vitro. We analyzed changes in Grb10 DNA methylation and histone modifications in serially passaged adult mouse tail-tip fibroblast cells with bisulfite genomic sequencing method and chromatin immunoprecipitation (ChIP) respectively. There was a significant reduction in global DNA methylation, but no changes in methylation at CpG island 1 (CGI1) of Grb10. There were no changes in methylation at histone H3K4me2, but less methylated histone H3K27me3 was detected, along with more of the acetylated histones H3Ac and H4Ac. The major-type transcript level of Grb10 in fibroblasts increased mildly after extended cultivation. These results suggest consecutive passaging may affect epigenetic modifications of Grb10 in adult fibroblast cells. (C) 2012 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：2699 / 2705
页数：7
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