Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

被引：9
作者：
Chow, Kelsey S. [1 ,2 ]
Jun, DuHyun [1 ,2 ]
Helm, Karen M. [3 ]
Wagner, David H. [1 ,2 ,4 ]
Majka, Susan M. [1 ,2 ,3 ]
机构：
[1] Univ Colorado Denver, Charles C Gates Regenerat Med & Stem Cell Biol Pr, Denver, CO 80204 USA
[2] Univ Colorado Denver, Dept Med, Denver, CO USA
[3] Univ Colorado Denver, Ctr Canc, Denver, CO USA
[4] Univ Colorado Denver, Webb Waring Inst, Denver, CO USA
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2011年 / 56期
关键词：
Developmental Biology; Issue; 56; mesenchymal stem cell; MSC; lung stem cell; resident lung mesenchymal stem cells; stem cells;
D O I：
10.3791/3159
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Tissue resident mesenchymal stem cells (MSC) are important regulators of tissue repair or regeneration, fibrosis, inflammation, angiogenesis and tumor formation. Taken together these studies suggest that resident lung MSC play a role during pulmonary tissue homeostasis, injury and repair during diseases such as pulmonary fibrosis (PF) and arterial hypertension (PAH). Here we describe a technology to define a population of resident lung MSC. The definition of this population in vivo pulmonary tissue using a define set of markers facilitates the repeated isolation of a well-characterized stem cell population by flow cytometry and the study of a specific cell type and function.
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页数：6
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