Soluble enzyme cross-linking via multi-component reactions: a new generation of cross-linked enzymes

被引：29
作者：
Ashjari, Maryam [1 ]
Garmroodi, Maryam [1 ]
Ahrari, Faezeh [1 ,2 ]
Yousefi, Maryam [3 ]
Mohammadi, Mehdi [1 ]
机构：
[1] Natl Inst Genet Engn & Biotechnol NIGEB, Inst Ind & Environm Biotechnol, Bioproc Engn Dept, Tehran, Iran
[2] Shahid Beheshti Univ, Fac Chem, Dept Pure Chem, GC, Tehran, Iran
[3] ACECR, Avicenna Res Inst, Nanobiotechnol Res Ctr, Tehran, Iran
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 67期
基金：
美国国家科学基金会;
关键词：
RHIZOMUCOR-MIEHEI LIPASE; BOVINE SERUM-ALBUMIN; COVALENT IMMOBILIZATION; ENANTIOSELECTIVE RESOLUTION; COMPONENT REACTION; CANDIDA-RUGOSA; SUPPORTS; CLEAS; OPTIMIZATION; DESIGN;
D O I：
10.1039/d0cc03429k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
By using a isocyanide-based multi-component reaction for the immobilization of the soluble forms ofRhizomucor mieheilipase (RML) andThermomyces lanuginosalipase (TLL), the first step of enzyme aggregation or crystallization in the traditional methods of cross-linking was bypassed. High immobilization yields and specific activities were achieved for both lipases.
引用
收藏
页码：9683 / 9686
页数：4
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