Decreased Immunoreactivities of the Chloride Transporters, KCC2 and NKCC1, in the Lateral Superior Olive Neurons of Kanamycin-treated Rats

被引:1
作者
Suh, Myung-Whan [2 ]
Ahn, Seung Cheol [1 ]
机构
[1] Dankook Univ, Coll Med, Dept Physiol, Cheonan 330714, South Korea
[2] Dankook Univ, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Cheonan 330714, South Korea
关键词
KCC2; NKCC1; Lateral superior olive; Kanamycin; Cochlea; AUDITORY BRAIN-STEM; SYNAPTIC-TRANSMISSION; CIRCLING MOUSE; HIPPOCAMPAL-NEURONS; GABA; COTRANSPORTER; EXPRESSION; MATURATION; SYNAPSES; DEAFNESS;
D O I
10.3342/ceo.2012.5.3.117
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Objectives. From our previous study about the weak expressions of potassium-chloride (KCC2) and sodium-potassium-2 chloride (NKCC1) co-transporters in the lateral superior olive (LSO) in circling mice, we hypothesized that partially damaged cochlea of circling mice might be a cause of the weak expressions of KCC2 or NKCC1. To test this possibility, we reproduced the altered expressions of KCC2 and NKCC1 in the LSO of rats, whose cochleae were partially destroyed with kanamycin. Methods. Rat pups were treated with kanamycin from postnatal (P)3 to P8 (700 mg/kg, subcutaneous injection, twice a day) and sacrificed for immunohistochemical analysis, scanning electron microscope (SEM) and auditory brain stem response. Results. The SEM study revealed partially missing hair cells in P9 rats treated with kanamycin, and the hearing threshold was elevated to 63.8 +/- 2.5 dB SPL (4 ears) at P16. Both KCC2 and NKCC1 immunoreactivities were more prominent in control rats on P16. On 9 paired slices, the mean densities of NKCC1 immunoreactivities were 118.0 +/- 1.0 (control) and 112.2 +/- 1.2 (kanamycin treated), whereas those of KCC2 were 115.7 +/- 1.5 (control) and 112.0 +/- 0.8 (kanamycin treated). Conclusion. We concluded that weak expressions of KCC2 and NKCC1 in circling mice were due to partial destruction of cochleae.
引用
收藏
页码:117 / 121
页数:5
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