A novel medium for the oosporogenesis of Aphanomyces cochlioides

被引：1
|
作者：
Paternoster, MP [1 ]
Burns, RG [1 ]
机构：
[1] UNIV KENT,RES SCH BIOSCI,CANTERBURY CT2 7NJ,KENT,ENGLAND
来源：
MYCOLOGICAL RESEARCH | 1996年 / 100卷
基金：
英国生物技术与生命科学研究理事会;
关键词：
D O I：
10.1016/S0953-7562(96)80044-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Reliable production of infective spores is an essential requirement in the screening of putative biological control agents. We report the development of a novel and simple medium for the production of viable oospores of Aphanomyces cochlioides, one of the principal causes of damping-off of sugar beet. The semi-solid medium consists of 5 g l(-1) corn meal agar and 1 g l(-1) mycological peptone and supports the development of 2.25 x 10(5) oospores cm(-2) in 5 d. In a plant bioassay for post-emergence damping-off, the oospores had a consistent pathogenic effect with 50+/-5.7% damping-off induced by 170 oospores g(-1) soil (-15 kPa matric potential) after 20 d. Higher numbers (1700 g(-1) soil) produced 88.8+/-9.1% disease in emerged plants after 20 d.
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页码：936 / 938
页数：3
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