Enhancing neutralizing antibody production by an interferon-inducing porcine reproductive and respiratory syndrome virus strain

被引:41
作者
Wang, Rong [1 ,2 ]
Xiao, Yueqiang [1 ,2 ,3 ]
Opriessnig, Tanja [4 ]
Ding, Yi [5 ]
Yu, Ying [1 ,2 ]
Nan, Yuchen [1 ,2 ]
Ma, Zexu [1 ,2 ]
Halbur, Patrick G. [4 ]
Zhang, Yan-Jin [1 ,2 ]
机构
[1] Univ Maryland, Mol Virol Lab, VA MD Reg Coll Vet Med, College Pk, MD 20742 USA
[2] Univ Maryland, Maryland Pathogen Res Inst, College Pk, MD 20742 USA
[3] Shandong Binzhou Anim Sci & Vet Med, Binzhou, Shandong, Peoples R China
[4] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA
[5] Univ Maryland, Dept Anim Sci, College Pk, MD 20742 USA
基金
英国生物技术与生命科学研究理事会;
关键词
Porcine reproductive and respiratory syndrome virus; PRRSV; Neutralizing antibody; IFN-inducing A2MC2; SYNDROME PRRS VIRUS; LELYSTAD VIRUS; MONOCLONAL-ANTIBODIES; PASSIVE TRANSFER; CELL-LINE; T-CELL; REPLICATION; INFECTION; VACCINE; ISOLATE;
D O I
10.1016/j.vaccine.2013.09.023
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) continues to cause substantial economic losses to the global swine industry. PRRSV appears to inhibit synthesis of type I interferons (IFNs), such as IFN-alpha and -beta, which are critical for the innate immunity and play an important role in the modulation of adaptive immunity. An atypical PRRSV strain, A2MC2, is able to induce type I IFNs in vitro. In this study, A2MC2 induction of neutralizing antibodies in vivo was compared with the Ingelvac PRRS modified live virus (MLV) vaccine strain and VR-2385 (a moderate virulent strain). Three-week-old pigs were exposed to these PRRSV strains via intranasal or intramuscular routes to also account for a possible effect of inoculation routes. The interferon-inducing A2MC2 resulted in earlier onset and significantly higher levels of PRRSV neutralizing antibodies than the MLV. In addition, the A2MC2-induced neutralizing antibodies were capable of neutralizing VR-2385, a heterologous strain. The pigs exposed via intranasal route had higher titers of neutralizing antibodies than those injected via intramuscular route. Macroscopic and microscopic lung lesions 14 days post-exposure indicated that A2MC2 had similar virulence in vivo as VR-2385. Pulmonary alveolar macrophages (PAMs) collected during the necropsy 14 days post-exposure in the A2MC2 group had higher level expression of IFN-gamma than the MLV group. These results indicate that A2MC2 can be further explored for development of an improved vaccine against PRRS. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5537 / 5543
页数:7
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