Physical and genetic map of the Bacteroides fragilis YCH46 chromosome

被引：8
作者：
Kuwahara, T
Sarker, MR
Ugai, H
Akimoto, S
Shaheduzzaman, SM
Nakayama, H
Miki, T
Ohnishi, Y
机构：
[1] Univ Tokushima, Dept Bacteriol, Sch Med, Tokushima 7708503, Japan
[2] Wakayama Med Univ, Dept Microbiol, Wakayama 6410012, Japan
来源：
FEMS MICROBIOLOGY LETTERS | 2002年 / 207卷 / 02期
关键词：
physical map; pulsed-field gel electrophoresis; Bacteroides fragilis;
D O I：
10.1111/j.1574-6968.2002.tb11050.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 Noti, seven Ascl, and six I-Ceul sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis. cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-Field get electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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页码：193 / 197
页数：5
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