Simultaneous 3D imaging of Morphology and Nanoparticle Distribution in Single Cells with the Cell-CT™ Technology

被引：5

作者：

Neumann, Thomas ^{[1
]}

Miao, Qin ^{[2
]}

Yu, Julia ^{[1
]}

Fauver, Mark ^{[1
]}

Meyer, Michael ^{[1
]}

Rahn, J. Richard ^{[1
]}

Lancaster, Christy A. ^{[1
]}

Seibel, Eric J. ^{[2
]}

Nelson, Alan C. ^{[1
]}

机构：

[1] VisionGate Inc, Gig Harbor, WA 98335 USA

[2] Univ Washington, Seattle, WA 98195 USA

来源：

2008 30TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-8 | 2008年

关键词：

D O I：

10.1109/IEMBS.2008.4649169

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The Cell-CT(TM) is an optical projection tomography microscope (OPTM) developed for high resolution 3D imaging of single cells based on absorption stains and brightfield microscopy. In this study we demonstrate the use of the Cell-CT(TM) in multi-color mode for simultaneous imaging of cellular 3D morphology and the 3D distribution of nanoparticle clusters in the cytoplasm. The ability to image cellular processes in relation to cellular compartments with a non-fluorescence 3D technology opens new perspectives for molecular research.

引用

页码：379 / 381

页数：3

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