Planar waveguides as high performance sensing platforms for fluorescence-based multiplexed oligonucleotide hybridization assays

被引：66

作者：

Budach, W ^{[1
]}

Abel, AP ^{[1
]}

Bruno, AE ^{[1
]}

Neuschäfer, D ^{[1
]}

机构：

[1] Novartis Pharma AG, DMPK, CH-4002 Basel, Switzerland

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 16期

关键词：

D O I：

10.1021/ac990092e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

16-Mer and 22-mer oligonucleotide capture probes with an amino function at the 5'-end were covalently immobilized on (3-glycidoxypropyl)trimethoxysilane (GOPTS) silanized planar waveguides in "checkerboard"-style 2-dimensional arrays by means of ink-jet printing in order to demonstrate the potential of multiplexed planar waveguide biosensing. The laser-induced fluorescence was collected by a CCD camera, providing quantitative information regarding the intensity of individual sensing pads. In model hybridization assays with Cy5-labeled complementary oligonucleotides, sample concentrations down to 50 fM were successfully detected within 12 min. The overall spot-to-spot variability of the ink-jet immobilization in combination with amino functionalized oligonucleotides and GOPTS silanized waveguides was found to be about 12% and almost independent of the formation of an equilibrium between the labeled analyte and capture probes. Both pipet-made and ink-jet-made oligonucleotide spots showed comparable assay performances.

引用

页码：3347 / 3355

页数：9

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