New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions

被引：92

作者：

Schirrmeister, Joerg F. ^{[1
]}

Liebenow, Anna-Lisa

Pelz, Klaus ^{[2
]}

Wittmer, Annette ^{[2
]}

serr, Annerose ^{[2
]}

Hellwig, Elmar

Al-Ahmad, Ali

机构：

[1] Univ Freiburg, Univ Clin & Dent Hosp, Univ Sch & Dent Hosp, Dept Operat Dent & Periodontol, D-79106 Freiburg, Germany

[2] Univ Freiburg, Dept Microbiol & Hyg, Inst Med Microbiol & Hyg, D-79106 Freiburg, Germany

来源：

JOURNAL OF ENDODONTICS | 2009年 / 35卷 / 02期

基金：

俄罗斯科学基金会;

关键词：

16S rRNA; endodontic microbiology; endodontic retreatment; molecular biology; Vagococcus fluvialis; POLYMERASE-CHAIN-REACTION; FAILED ENDODONTIC TREATMENT; ENTEROCOCCUS-FAECALIS; SOLOBACTERIUM-MOOREI; APICAL PERIODONTITIS; PERIAPICAL LESIONS; NESTED PCR; INFECTIONS; MICROORGANISMS; CANALS;

D O I：

10.1016/j.joen.2008.10.024

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing. Microorganisms were detected in 10 of 18 teeth. The majority of positive samples revealed a mixed culture of 2-8 species. In 2 teeth Enterococcus faecalis was the only detected species. For the first time Vagococcus fluvialis was detected in root canals. Solobacterium moorei and Fusobacterium nucleatum were the most prevalent species. Presence of F. nucleatum, was associated with the presence of S. moorei in 5 of 7 cases. In all teeth with Parvimonas micra and Dialister invisus, F. nucleatum and S. moorei were found. Moreover, members of additional different genera were detected delivering bacterial compositions that have been not described yet. (J Endod 2009;35:169-174)

引用

页码：169 / 174

页数：6

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