The use of microalgae and cyanobacteria for the production of biofuels and other substances of commercial interest has been widely advertised as an extremely promising sustainable technology, due to the high areal productivity, potential for fixation of CO2, possibility of using non-arable land and alternative sources of nutrients such as brackish water and agricultural and industrial effluents. The commercial production of cyanobacteria in open raceway ponds was studied through the combination of a mathematical model for the algal growth with technical, economical and sustainability evaluations. A macromodel was developed to simulate the ponds, and it was used to assess the impact of environmental variables, such as light and temperature, and to optimize the process conditions for operation and harvesting. A detailed economic analysis demonstrated the impact of capital, operation costs and energy consumption, also highlighting the importance of revenue from high value products to process viability, considering the current technology. The sensitivity analysis and evaluation of both technology improvements and alternative business models enabled the prioritization of future research areas, based on economic and environmental impact.