Transmembrane Protein Activation Refined by Site-Specific Hydration Dynamics

被引：46
|
作者：
Hussain, Sunyia [1 ,2 ]
Franck, John M. [1 ,2 ]
Han, Songi [1 ,2 ]
机构：
[1] Univ Calif Santa Barbara, Dept Chem Engn, Santa Barbara, CA 93016 USA
[2] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93016 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 07期
关键词：
dynamic nuclear polarization; EPR spectroscopy; hydration dynamics; membrane proteins; proteorhodopsin; E-F LOOP; NUCLEAR-POLARIZATION; MOLECULAR-DYNAMICS; INTERHELICAL LOOP; MEMBRANE-PROTEINS; WATER DYNAMICS; SPIN; BACTERIORHODOPSIN; STATE; PROTEORHODOPSIN;
D O I：
10.1002/anie.201206147
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Proteins on film: The Overhauser dynamic nuclear polarization method resolves hydration dynamics to an unprecedented level of detail for a transmembrane protein surface. The heterogeneous hydration landscape of proteorhodopsin rearranges upon photoactivation (see picture), thus providing an insight into how water contributes to protein function even for biological systems embedded in a hydrophobic membrane. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1953 / 1958
页数：6
相关论文
共 50 条
  • [41] Site-specific protein immobilization by Staudinger ligation
    1600, American Chemical Society (125):
  • [42] Site-specific hydration status of an amphipathic peptide in AOT reverse micelles
    Mukherjee, Smita
    Chowdhury, Pramit
    DeGrado, William F.
    Gai, Feng
    LANGMUIR, 2007, 23 (22) : 11174 - 11179
  • [43] Site-Specific Protein Dynamics Probed by Ultrafast Infrared Spectroscopy of a Noncanonical Amino Acid
    Hall, Christopher R.
    Collado, Jinnette Tolentino
    Iuliano, James N.
    Gil, Agnieszka A.
    Adamczyk, Katrin
    Lukacs, Andras
    Greetham, Gregory M.
    Sazanovich, Igor
    Tonge, Peter J.
    Meech, Stephen R.
    JOURNAL OF PHYSICAL CHEMISTRY B, 2019, 123 (45): : 9592 - 9597
  • [44] Advances in Solid-State Relaxation Methodology for Probing Site-Specific Protein Dynamics
    Lewandowski, Jozef Romuald
    ACCOUNTS OF CHEMICAL RESEARCH, 2013, 46 (09) : 2018 - 2027
  • [45] Fast Pressure Jump Reveals Site-Specific Protein Dehydration-Folding Dynamics
    Prigozhin, Maxim B.
    Zhang, Yi
    Schulten, Klaus J.
    Gruebele, Martin
    Pogorelov, Taras V.
    BIOPHYSICAL JOURNAL, 2019, 116 (03) : 38A - 38A
  • [46] Gene activation in plastids by the CRE site-specific recombinase
    Tungsuchat, Tarinee
    Kuroda, Hiroshi
    Narangajavana, Jarunya
    Maliga, Pal
    PLANT MOLECULAR BIOLOGY, 2006, 61 (4-5) : 711 - 718
  • [47] Quantitative Site-specific Phosphorylation Dynamics of Human Protein Kinases during Mitotic Progression
    Dulla, Kalyan
    Daub, Henrik
    Hornberger, Renate
    Nigg, Erich A.
    Koerner, Roman
    MOLECULAR & CELLULAR PROTEOMICS, 2010, 9 (06) : 1167 - 1181
  • [48] Gene activation in plastids by the CRE site-specific recombinase
    Tarinee Tungsuchat
    Hiroshi Kuroda
    Jarunya Narangajavana
    Pal Maliga
    Plant Molecular Biology, 2006, 61 : 711 - 718
  • [49] Asymmetric activation of Xer site-specific recombination by FtsK
    Massey, TH
    Aussel, L
    Barre, FX
    Sherratt, DJ
    EMBO REPORTS, 2004, 5 (04) : 399 - 404
  • [50] Structure of the Influenza C virus CM2 protein transmembrane domain obtained by site-specific infrared dichroism and global molecular dynamics searching
    Kukol, A
    Arkin, IT
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (06) : 4225 - 4229
12345