The β2 subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney

被引：9
作者：
Behrends, S
Budaeus, L
Kempfert, J
Scholz, H
Starbatty, J
Vehse, K
机构：
[1] Univ Hamburg, Inst Expt, D-20251 Hamburg, Germany
[2] Univ Hamburg, Klin Pharmakol, D-20251 Hamburg, Germany
来源：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | 2001年 / 364卷 / 06期
关键词：
kidney; development; soluble guanylyl cyclase; nitric oxide;
D O I：
10.1007/s00210-001-0499-5
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
We have recently shown that nitric oxide activates the beta (2) subunit of soluble guanylyl cyclase. In the present study, we show developmental regulation of this subunit. Analysis of mRNA expression by RT-PCR and RNase protection analysis in kidneys revealed no expression of the beta (2) subunit in neonatal and strong expression in adult rats. A reciprocal regulation with much lower expression levels was observed in rat lung. Further examination of kidneys from 3, 6, 16, 22, 25, 31 and 36-day-old rats showed that significant expression appears between postnatal day 16 and 22. Isolation of the rat beta (2) promoter by genome walking and cloning into a reporter gene vector showed promoter activity for the sense but not the antisense construct providing an in vitro assay for further analysis of the developmental beta (2) subunit regulation.
引用
收藏
页码：573 / 576
页数：4
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