Amplified detection of genome-containing biological targets using terminal deoxynucleotidyl transferase-assisted rolling circle amplification

被引：39

作者：

Du, Yi-Chen ^{[1
]}

Zhu, Yan-Jun ^{[1
]}

Li, Xiao-Yu ^{[1
]}

Kong, De-Ming ^{[1
,2
]}

机构：

[1] Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

[2] Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 06期

基金：

中国国家自然科学基金;

关键词：

TUMOR-MARKER; CAPTURE; CHALLENGES; BIOSENSORS; STRATEGY; APTAMER; RELEASE;

D O I：

10.1039/c7cc09337c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We proposed a terminal deoxynucleotidyl transferase (TdT)-assisted rolling circle amplification (RCA) strategy for the amplified detection of genome-containing biological targets. The synergetic signal amplification of DNase I-mediated genomic DNA fragmentation and subsequent RCA allow the sensing platform to have extraordinarily high sensitivity.

引用

页码：682 / 685

页数：4

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