A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine

被引：57

作者：

Wang, Liguo ^{[1
]}

Zhou, Qi ^{[1
]}

Zhu, Baocun ^{[1
]}

Yan, Liangguo ^{[1
]}

Ma, Zhenmin ^{[1
]}

Du, Bin ^{[1
]}

Zhang, Xiaoling ^{[2
]}

机构：

[1] Univ Jinan, Sch Resources & Environm, Jinan 250022, Peoples R China

[2] Beijing Inst Technol, Sch Chem, Dept Chem, Key Lab Cluster Sci,Minist Educ, Beijing 100081, Peoples R China

来源：

DYES AND PIGMENTS | 2012年 / 95卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Colorimetric; Fluorescent chemodosimeter; Cysteine; Homocysteine; Simultaneous detection; Fluorescein; LIVING CELLS; PROBE; THIOLS; RED; CHEMOSENSOR; SENSOR; DIKETOPYRROLOPYRROLE; BIOTHIOLS; SHIFT;

D O I：

10.1016/j.dyepig.2012.05.006

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A highly selective, visible-light-excited and OFF ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a "naked-eye" indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 mu M (for Cys) and 0.8 mu M (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：275 / 279

页数：5

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