A high-throughput approach for measuring temporal changes in the interactome

被引：250
作者：
Kristensen, Anders R. [1 ,2 ]
Gsponer, Joerg [1 ,2 ]
Foster, Leonard J. [1 ,2 ]
机构：
[1] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V5Z 1M9, Canada
[2] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V5Z 1M9, Canada
来源：
NATURE METHODS | 2012年 / 9卷 / 09期
基金：
加拿大创新基金会;
关键词：
PROTEIN-PROTEIN INTERACTIONS; IN-VIVO; COMPLEXES;
D O I：
10.1038/nmeth.2131
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Interactomes are often measured using affinity purification-mass spectrometry (AP-MSMS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MSMS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
引用
收藏
页码：907 / +
页数：5
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