A high-throughput approach for measuring temporal changes in the interactome

被引：262

作者：

Kristensen, Anders R. ^{[1
,2
]}

Gsponer, Joerg ^{[1
,2
]}

Foster, Leonard J. ^{[1
,2
]}

机构：

[1] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V5Z 1M9, Canada

[2] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V5Z 1M9, Canada

来源：

NATURE METHODS | 2012年 / 9卷 / 09期

基金：

加拿大创新基金会;

关键词：

PROTEIN-PROTEIN INTERACTIONS; IN-VIVO; COMPLEXES;

D O I：

10.1038/nmeth.2131

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interactomes are often measured using affinity purification-mass spectrometry (AP-MSMS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MSMS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.

引用

页码：907 / +

页数：5

共 24 条

[1] Proteomic characterization of the human centrosome by protein correlation profiling [J].

Andersen, JS ;

Wilkinson, CJ ;

Mayor, T ;

Mortensen, P ;

Nigg, EA ;

Mann, M .

NATURE, 2003, 426 (6966) :570-574

[2]

[Anonymous], 2006, 23 INT C MACH LEARN, DOI [DOI 10.1145/1143844.1143874, 10.1145/1143844.1143874]

[3] The IntAct molecular interaction database in 2010 [J].

Aranda, B. ;

Achuthan, P. ;

Alam-Faruque, Y. ;

Armean, I. ;

Bridge, A. ;

Derow, C. ;

Feuermann, M. ;

Ghanbarian, A. T. ;

Kerrien, S. ;

Khadake, J. ;

Kerssemakers, J. ;

Leroy, C. ;

Menden, M. ;

Michaut, M. ;

Montecchi-Palazzi, L. ;

Neuhauser, S. N. ;

Orchard, S. ;

Perreau, V. ;

Roechert, B. ;

van Eijk, K. ;

Hermjakob, H. .

NUCLEIC ACIDS RESEARCH, 2010, 38 :D525-D531

[4] Proteomic snapshot of the EGF-induced ubiquitin network [J].

Argenzio, Elisabetta ;

Bange, Tanja ;

Oldrini, Barbara ;

Bianchi, Fabrizio ;

Peesari, Raghunath ;

Mari, Sara ;

Di Fiore, Pier Paolo ;

Mann, Matthias ;

Polo, Simona .

MOLECULAR SYSTEMS BIOLOGY, 2011, 7

[5] STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes [J].

Bache, KG ;

Raiborg, C ;

Mehlum, A ;

Stenmark, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (14) :12513-12521

[6] Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics [J].

Blagoev, B ;

Ong, SE ;

Kratchmarova, I ;

Mann, M .

NATURE BIOTECHNOLOGY, 2004, 22 (09) :1139-1145

[7] Oncogenic kinase signalling [J].

Blume-Jensen, P ;

Hunter, T .

NATURE, 2001, 411 (6835) :355-365

[8] MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification [J].

Cox, Juergen ;

Mann, Matthias .

NATURE BIOTECHNOLOGY, 2008, 26 (12) :1367-1372

[9] Large-scale mapping of human protein-protein interactions by mass spectrometry [J].

Ewing, Rob M. ;

Chu, Peter ;

Elisma, Fred ;

Li, Hongyan ;

Taylor, Paul ;

Climie, Shane ;

McBroom-Cerajewski, Linda ;

Robinson, Mark D. ;

O'Connor, Liam ;

Li, Michael ;

Taylor, Rod ;

Dharsee, Moyez ;

Ho, Yuen ;

Heilbut, Adrian ;

Moore, Lynda ;

Zhang, Shudong ;

Ornatsky, Olga ;

Bukhman, Yury V. ;

Ethier, Martin ;

Sheng, Yinglun ;

Vasilescu, Julian ;

Abu-Farha, Mohamed ;

Lambert, Jean-Philippe ;

Duewel, Henry S. ;

Stewart, Ian I. ;

Kuehl, Bonnie ;

Hogue, Kelly ;

Colwill, Karen ;

Gladwish, Katharine ;

Muskat, Brenda ;

Kinach, Robert ;

Adams, Sally-Lin ;

Moran, Michael F. ;

Morin, Gregg B. ;

Topaloglou, Thodoros ;

Figeys, Daniel .

MOLECULAR SYSTEMS BIOLOGY, 2007, 3 (1)

[10] A mammalian organelle map by protein correlation profiling [J].

Foster, LJ ;

de Hoog, CL ;

Zhang, YL ;

Zhang, Y ;

Xie, XH ;

Mootha, VK ;

Mann, M .

CELL, 2006, 125 (01) :187-199

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