Long-Term Fluorescent Cellular Tracing by the Aggregates of AIE Bioconjugates

被引：347

作者：

Wang, Zhengke ^{[1
,2
,3
,5
]}

Chen, Sijie ^{[1
,2
,5
]}

Lam, Jacky W. Y. ^{[1
,2
,5
]}

Qin, Wei ^{[1
,2
]}

Kwok, Ryan T. K. ^{[1
,2
]}

Xie, Ni ^{[1
,2
]}

Hu, Qiaoling ^{[3
]}

Tang, Ben Zhong ^{[1
,2
,4
,5
]}

机构：

[1] Hong Kong Univ Sci & Technol, Inst Adv Study, Div Biomed Engn, Dept Chem, Kowloon, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Inst Mol Funct Mat, Kowloon, Hong Kong, Peoples R China

[3] Zhejiang Univ, MoE Key Lab Macromol Synth & Functionalizat, Inst Biomed Macromol, Hangzhou 310027, Peoples R China

[4] SCUT, State Key Lab Luminescent Mat & Devices, Guangdong Innovat Res Team, SCUT HKUST Joint Res Lab, Guangzhou 510640, Guangdong, Peoples R China

[5] HKUST Shenzhen Res Inst, Shenzhen 518057, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 22期

关键词：

INDUCED EMISSION; PHOTOPHYSICAL PROPERTIES; CHITOSAN NANOPARTICLES; MOLECULES; ENHANCEMENT; POLYMERS; PROBES; PHOTOLUMINESCENCE; TETRAPHENYLETHENE; FLUOROGEN;

D O I：

10.1021/ja312581r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

There is a great demand for long-term cellular tracers because of their great importance in monitoring biological processes, pathological pathways, therapeutic effects, etc. Herein we report a new type of fluorescence turn-on probe for tracing live cells over a long period of time. We synthesized the fluorogenic probe by attaching a large number of tetraphenylethene (TPE) labels to a chitosan (CS) chain. The resultant TPE-CS bioconjugate shows a unique aggregation-induced emission (AIE) behavior. It is nonfluorescent when dissolved but becomes highly emissive when its molecules are aggregated. The AIE aggregates can be readily internalized by HeLa cells. The cellular staining by the TPE-CS aggregates is so indelible that it enables cell tracing for as long as 15 passages. The internalized AIE aggregates are kept inside the cellular compartments and do not contaminate other cell lines in the coculture systems, permitting the differentiation of specific cancerous cells from normal healthy cells.

引用

页码：8238 / 8245

页数：8

共 88 条

[1] Enantioselective Sensing by Luminescence [J].

Accetta, Alessandro ;

Corradini, Roberto ;

Marchelli, Rosangela .

LUMINESCENCE APPLIED IN SENSOR SCIENCE, 2011, 300 :175-216

[2]

Akiyama S, 2012, J SYN ORG CHEM JPN, V70, P465

[3] Organic Solid-State Fluorescence: Strategies for Generating Switchable and Tunable Fluorescent Materials [J].

Anthony, Savarimuthu Philip .

CHEMPLUSCHEM, 2012, 77 (07) :518-531

[4] Practical synthesis of unsymnetrical tetraarylethylenes and their application for the preparation of [triphenylethylene-spacer-triphenylethylene] triads [J].

Banerjee, Moloy ;

Emond, Susanna J. ;

Lindeman, Sergey V. ;

Rathore, Rajendra .

JOURNAL OF ORGANIC CHEMISTRY, 2007, 72 (21) :8054-8061

[5] Emission enhancement by formation of aggregates in hybrid chromophoric surfactant amphiphile/silica nanocomposites [J].

Bhongale, CJ ;

Hsu, CS .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (09) :1404-1408

[6]

Bunau G. V., 1970, JB BIRKSPHOTOPHYSICS, V74, P1294

[7]

Chan CPY, 2009, BIOANALYSIS, V1, P115, DOI [10.4155/bio.09.15, 10.4155/BIO.09.15]

[8] Silole-containing polymers: Chemistry and optoelectronic properties [J].

Chen, Junwu ;

Cao, Yong .

MACROMOLECULAR RAPID COMMUNICATIONS, 2007, 28 (17) :1714-1742

[9] Recent advances in organic mechanofluorochromic materials [J].

Chi, Zhenguo ;

Zhang, Xiqi ;

Xu, Bingjia ;

Zhou, Xie ;

Ma, Chunping ;

Zhang, Yi ;

Liu, Siwei ;

Xu, Jiarui .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (10) :3878-3896

[10]

Christian Weinand, 2008, Journal of Biomedical Science & Engineering, V1, P163, DOI 10.4236/jbise.2008.13027

← 1 2 3 4 5 6 7 8 9 →