Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment

被引:53
作者
Pelikan, Claus [1 ,2 ]
Wasmund, Kenneth [1 ,2 ]
Glombitza, Clemens [3 ,4 ]
Hausmann, Bela [1 ,5 ,6 ,7 ]
Herbold, Craig W. [1 ]
Flieder, Mathias [1 ]
Loy, Alexander [1 ,2 ,5 ,6 ]
机构
[1] Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Vienna, Austria
[2] Austrian Polar Res Inst, Vienna, Austria
[3] Aarhus Univ, Ctr Geomicrobiol, Dept Biol, Aarhus, Denmark
[4] Swiss Fed Inst Technol, Dept Environm Syst Sci, Ziirich, Switzerland
[5] Med Univ Vienna, Joint Microbiome Facil, Vienna, Austria
[6] Univ Vienna, Vienna, Austria
[7] Med Univ Vienna, Dept Lab Med, Vienna, Austria
基金
奥地利科学基金会;
关键词
MULTIPLE SEQUENCE ALIGNMENT; PARTICULATE ORGANIC-MATTER; CHEMICAL-COMPOSITION; CRENARCHAEOTAL GROUP; SINGLE-CELL; FATTY-ACIDS; GEN; NOV; DATABASE; TOOL; TEMPERATURE;
D O I
10.1038/s41396-020-00817-6
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade them remain poorly resolved. Here, we revealed identities, trophic interactions, and genomic features of bacteria that degraded C-13-labeled proteins and lipids in cold anoxic microcosms containing sulfidic subarctic marine sediment. Supplemented proteins and lipids were rapidly fermented to various volatile fatty acids within 5 days. DNA-stable isotope probing (SIP) suggested Psychrilyobacter atlanticus was an important primary degrader of proteins, and Psychromonas members were important primary degraders of both proteins and lipids. Closely related Psychromonas populations, as represented by distinct 16S rRNA gene variants, differentially utilized either proteins or lipids. DNA-SIP also showed C-13-labeling of various Deltaproteobacteria within 10 days, indicating trophic transfer of carbon to putative sulfate-reducers. Metagenome-assembled genomes revealed the primary hydrolyzers encoded secreted peptidases or lipases, and enzymes for catabolism of protein or lipid degradation products. Psychromonas species are prevalent in diverse marine sediments, suggesting they are important players in organic carbon processing in situ. Together, this study provides new insights into the identities, functions, and genomes of bacteria that actively degrade abundant necromass macromolecules in the seafloor.
引用
收藏
页码:833 / 847
页数:15
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