Adsorption of nisin on raw montmorillonite

被引:27
作者
Ibarguren, Carolina [1 ,2 ,3 ]
Naranjo, Pablo M. [1 ]
Stoetzel, Christian [2 ]
Carina Audisio, M. [1 ,4 ]
Sham, Edgardo L. [1 ,4 ]
Farfan Torres, E. Monica [1 ,5 ]
Mueller, Frank A. [2 ]
机构
[1] Univ Nacl Salta, Inst Invest Ind Quim, Consejo Nacl Invest Cient & Tecn INIQUI CONICET, Salta, Argentina
[2] Univ Jena, Inst Mat Sci & Technol IMT, D-07743 Jena, Germany
[3] Univ Nacl Salta, Fac Ciencias Salud, Salta, Argentina
[4] Univ Nacl Salta, Fac Ingn, Salta, Argentina
[5] Univ Nacl Salta, Fac Ciencias Exactas, Salta, Argentina
关键词
Nisin; Montmorillonite; Peptide/support interaction; Food biopreservative; ANTIMICROBIAL ACTIVITY; GENERAL TREATMENT; BACTERIOCIN; ORGANOCLAYS; CLAYS; PARANITROPHENOL; CLASSIFICATION; LIPOSOMES; SURFACES; ISOTHERM;
D O I
10.1016/j.clay.2013.12.031
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The aim of the present work was to develop food-grade materials capable of releasing antimicrobial agents. Montmorillonite (Mt), a raw, abundant inexpensive clay mineral approved as a food additive, was used as support material. The immobilized antimicrobial agent was pure nisin (Danisco, UK). A series of antibacterial compounds were prepared, and the antimicrobial activity of the resultant montmorillonite-nisin (MtNis) immobilized systems was determined against Enterococcus faecium Cl. The characterization of the antimicrobial powders showed evidences of nisin adsorption on by means of a "frustrated intercalation" model with a nisin loading (NL) saturation value close to 0.24 CEC (cation exchange capacity) of Mt. The antibacterial powders with NL above this value completely inhibited E. faecium Cl during 24 h of contact These results are encouraging for the development of nisin-based bioprotectors immobilized on raw Mt. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:88 / 95
页数:8
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